| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCSP |
| Uniprot No | P49901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 活性数据 | MCDQTKHSKCCPAKGNQCCPPQQNQCCQSKGNQCCPPKQNQCCQPKGSQCCPPKHNHCCQPKPPCCIQARCCGLETKPEVSPLNMESEPNSPQTQDKGCQTQQQPHSPQNESRPSK |
| 分子量 | 39.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MCSP蛋白的3篇参考文献(注：以下内容为模拟生成，实际文献可能需要通过数据库检索)：
1. **文献名称**：*Production and characterization of recombinant human MCSP for melanoma immunotherapy*
**作者**：Zhang Y, et al.
**摘要**：研究团队通过哺乳动物细胞表达系统成功克隆并纯化重组人MCSP蛋白，验证其与抗MCSP单克隆抗体的特异性结合，为基于MCSP的疫苗开发提供基础。
2. **文献名称**：*MCSP-mediated signaling pathways in melanoma cell invasion: Insights from recombinant protein studies*
**作者**：Johnson R, et al.
**摘要**：利用重组MCSP蛋白探究其在黑色素瘤细胞中的功能，发现MCSP通过与整合素相互作用激活MAPK通路，促进肿瘤细胞的迁移和侵袭。
3. **文献名称**：*Biochemical analysis of recombinant MCSP glycosylation variants and their immunogenicity*
**作者**：Lee S, et al.
**摘要**：通过不同表达系统(如CHO细胞和昆虫细胞)制备重组MCSP，分析糖基化修饰差异对蛋白稳定性和免疫原性的影响，为优化治疗性抗体设计提供数据支持。
如需具体文献，建议通过PubMed或Google Scholar以“recombinant human MCSP protein”为关键词检索近年研究。
Recombinant human MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan) protein is a genetically engineered version of a transmembrane glycoprotein highly expressed on the surface of melanoma cells and some other cancers. MCSP, also known as CSPG4 or HMW-MAA, plays critical roles in cell adhesion, migration, and signaling through interactions with extracellular matrix components. Its overexpression in malignant melanocytes correlates with tumor progression, metastasis, and resistance to therapy, making it a potential therapeutic target and diagnostic marker.
The recombinant MCSP protein is typically produced in mammalian or insect expression systems to preserve post-translational modifications and functional epitopes. It enables studies on MCSP's molecular mechanisms in tumor biology, particularly its involvement in PI3K/AKT and FAK pathways that drive cell proliferation and survival. Researchers utilize this protein to develop MCSP-targeted immunotherapies, including monoclonal antibodies, CAR-T cells, and cancer vaccines. Additionally, it serves as a critical reagent for antibody validation, biomarker discovery, and mechanistic studies exploring MCSP's role in modulating tumor microenvironment interactions. Recent investigations also explore its expression in non-melanoma malignancies, expanding its relevance in oncology research.
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