| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM10 |
| Uniprot No | Q7L590 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-874aa |
| 活性数据 | MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEADDGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPEKSPRPPLKERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSAPSQPLQTISRNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQIKEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTVVGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK |
| 分子量 | 124.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MCM10蛋白的3篇代表性文献(虚拟示例，仅供格式参考)：
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1. **文献名称**：*MCM10 regulates DNA replication elongation by promoting the assembly of the CMG helicase*
**作者**：Smith et al.
**摘要**：研究通过表达重组人MCM10蛋白，发现其作为支架促进MCM2-7复合物与GINS的结合，从而激活CMG解旋酶活性，在DNA复制叉延伸中起关键作用。
2. **文献名称**：*Structural and functional analysis of human MCM10 in replisome assembly*
**作者**：Zhang et al.
**摘要**：利用重组人MCM10蛋白的晶体结构解析，揭示其N端结构域结合双链DNA，C端结构域招募DNA聚合酶α，阐明了其在复制起始中的双重功能。
3. **文献名称**：*MCM10 overexpression as a biomarker for early-stage cancer detection*
**作者**：Li et al.
**摘要**：通过重组蛋白实验发现，MCM10在癌细胞中异常高表达，可通过血清检测其水平，提示其作为早期癌症诊断标志物的潜力。
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注：以上为模拟内容，实际文献需通过PubMed或Web of Science检索关键词“recombinant human MCM10”获取。
Recombination human MCM10 (mini-chromosome maintenance 10) protein is a critical component of the DNA replication machinery in eukaryotic cells. As part of the conserved MCM family, MCM10 plays essential roles in initiating and regulating DNA replication. It facilitates the assembly and activation of the CMG (Cdc45-MCM-GINS) helicase complex, ensuring efficient unwinding of DNA duplexes during replication origin firing. MCM10 also stabilizes replication forks and interacts with other replication factors like DNA polymerase α and the single-stranded DNA-binding protein RPA, thereby promoting replication fidelity and genome stability. Dysregulation of MCM10 is linked to replication stress, genomic instability, and cancer progression.
Recombinant human MCM10 protein is engineered for in vitro studies using expression systems such as E. coli or insect cells, often fused with affinity tags (e.g., His-tag) for purification. Its recombinant form enables detailed biochemical and structural analyses, including helicase activation mechanisms, replication origin licensing, and protein interaction networks. Research leveraging recombinant MCM10 has advanced understanding of replication restart, replication-coupled DNA repair, and cell cycle checkpoints. Additionally, it serves as a tool for screening anticancer drugs targeting replication vulnerabilities. Studies also explore its potential diagnostic value as a biomarker for cancer prognosis due to its overexpression in malignancies like glioblastoma and hepatocellular carcinoma.
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