| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCRS1 |
| Uniprot No | Q96EZ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 活性数据 | MDKDSQGLLD SSLMASGTAS RSEDEESLAG QKRASSQALG TIPKRRSSSR FIKRKKFDDE LVESSLAKSS TRAKGASGVE PGRCSGSEPS SSEKKKVSKA PSTPVPPSPA PAPGLTKRVK KSKQPLQVTK DLGRWKPADD LLLINAVLQT NDLTSVHLGV KFSCRFTLRE VQERWYALLY DPVISKLACQ AMRQLHPEAI AAIQSKALFS KAEEQLLSKV GSTSQPTLET FQDLLHRHPD AFYLARTAKA LQAHWQLMKQ YYLLEDQTVQ PLPKGDQVLN FSDAEDLIDD SKLKDMRDEV LEHELMVADR RQKREIRQLE QELHKWQVLV DSITGMSSPD FDNQTLAVLR GRMVRYLMRS REITLGRATK DNQIDVDLSL EGPAWKISRK QGVIKLKNNG DFFIANEGRR PIYIDGRPVL CGSKWRLSNN SVVEIASLRF VFLINQDLIA LIRAEAAKIT PQ |
| 分子量 | 51,8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为示例，建议通过学术数据库(如PubMed、Google Scholar)以“recombinant human MCRS1 protein”或“MCRS1 functional study”等关键词检索获取真实文献：
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1. **文献名称**: *MCRS1 modulates telomerase reverse transcriptase activity and promotes cell proliferation*
**作者**: Jiang et al. (2006)
**摘要**: 研究证实重组人MCRS1蛋白直接结合端粒酶逆转录酶(TERT)，通过稳定其活性促进癌细胞增殖，提示其在端粒调控中的作用。
2. **文献名称**: *MCRS1 interacts with the c-Jun/JNK pathway to drive oncogenic transformation*
**作者**: Lin et al. (2008)
**摘要**: 通过重组表达MCRS1蛋白发现其激活JNK/c-Jun信号通路，促进细胞周期进展和肿瘤发生，揭示其在癌症中的分子机制。
3. **文献名称**: *Structural and functional analysis of recombinant human MCRS1 in chromatin remodeling*
**作者**: Chen et al. (2015)
**摘要**: 利用重组MCRS1蛋白解析其结构，发现其通过结合染色质修饰复合体调控基因表达，为表观遗传机制提供依据。
4. **文献名称**: *Recombinant MCRS1 mediates 53BP1 recruitment and DNA damage response*
**作者**: Smith et al. (2012)
**摘要**: 研究表明重组MCRS1蛋白参与DNA损伤修复，通过招募53BP1至损伤位点，影响细胞对放射治疗的敏感性。
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**注**：上述为示例文献，实际文献需通过学术平台检索。如需具体文献，可进一步提供研究方向(如癌症机制、结构生物学等)辅助精准查找。
Recombinant human MCRS1 (Microspherule Protein 1) is a multifunctional protein encoded by the MCRS1 gene. Structurally, it contains coiled-coil domains facilitating interactions with diverse partners. MCRS1 plays critical roles in cellular processes including transcription regulation, chromatin remodeling, mitotic spindle assembly, and cell cycle control. It is a core subunit of the INO80 chromatin remodeling complex, impacting gene expression, DNA repair, and replication. Additionally, MCRS1 interacts with the NuRD complex to modulate histone deacetylase activity and participates in telomerase assembly via its association with dyskerin.
Aberrant MCRS1 expression is linked to diseases. Overexpression is observed in various cancers, correlating with genomic instability and tumorigenesis. Studies suggest MCRS1 promotes cancer cell proliferation by regulating p53 and Rb pathways. It also influences stem cell self-renewal and senescence through telomerase-related mechanisms. These diverse functions make recombinant MCRS1 protein valuable for studying its molecular interactions and developing targeted therapies. Produced via bacterial or eukaryotic expression systems, recombinant MCRS1 enables structural analysis, in vitro binding assays, and screening for inhibitors in cancer research. Ongoing investigations aim to elucidate its exact mechanisms in health and disease.
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