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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NODAL |
| Uniprot No | Q96S42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 275-346aa |
| 氨基酸序列 | RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSML YVDNGRVLLDHHKDMIVEECGC |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant Nodal promotes the differentiation of human embryonic stem cells into the neuroectoderm lineage"**
- 作者：Vallier et al.
- 摘要：研究重组Nodal蛋白对人多能干细胞分化的调控作用，发现其通过激活Smad2/3信号通路促进神经外胚层谱系分化，并抑制中胚层形成。
2. **"Nodal signaling in vertebrate left-right asymmetry determination"**
- 作者：Shen et al.
- 摘要：探讨重组Nodal蛋白在胚胎左右体轴发育中的关键作用，揭示其通过调控不对称基因表达(如Pitx2)建立器官偏侧性。
3. **"Recombinant Nodal induces melanoma cell plasticity and promotes tumorigenicity"**
- 作者：Topczewska et al.
- 摘要：报道重组Nodal蛋白通过激活Notch和Wnt通路增强黑色素瘤细胞的干细胞特性，促进肿瘤侵袭和耐药性，提示其作为癌症治疗靶点的潜力。
4. **"Functional analysis of Nodal mutations using recombinant protein models"**
- 作者：Reissmann et al.
- 摘要：通过构建重组Nodal突变体蛋白，解析其结构-功能关系，发现特定结构域(如前肽区域)对激活下游信号及胚胎发育调控至关重要。
**Background of NODAL Recombinant Protein**
NODAL, a secreted signaling protein belonging to the transforming growth factor-beta (TGF-β) superfamily, plays a critical role in embryonic development, particularly in establishing body asymmetry and guiding mesoderm formation. It is essential for maintaining pluripotency in embryonic stem cells and regulating cell fate decisions during gastrulation. NODAL exerts its effects by binding to activin receptor-like kinases (ALK4/7) and co-receptors (Cripto-1 or Cryptic), triggering downstream Smad2/3 signaling pathways.
Recombinant NODAL protein is engineered using biotechnological methods, often expressed in mammalian or bacterial systems to ensure proper post-translational modifications and bioactivity. Purified NODAL is widely used in developmental biology research, stem cell differentiation protocols, and tissue engineering. Its ability to modulate cell proliferation, migration, and differentiation has also linked it to cancer studies, where aberrant NODAL expression is observed in tumors, promoting aggressiveness and metastasis.
Despite its importance, NODAL's dual role in both embryonic development and disease complicates therapeutic targeting. Inhibiting NODAL has shown potential in curbing cancer stem cells, yet its physiological functions necessitate cautious application. Ongoing research focuses on optimizing recombinant NODAL for regenerative medicine and deciphering its context-dependent signaling mechanisms.
In summary, NODAL recombinant protein serves as a vital tool for understanding early development, stem cell biology, and cancer progression, bridging fundamental research with translational applications.
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