| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAN2B2 |
| Uniprot No | Q9Y2E5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1009aa |
| 活性数据 | MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPPDGVYPNMSEPVTPANINLYAEALVANVKQRAAWFRTPHVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGVRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYSIRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNRDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ |
| 分子量 | 140.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAN2B2蛋白的3篇参考文献示例，基于研究领域常见方向整理：
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1. **文献名称**：*Expression and characterization of recombinant human MAN2B2 in a mammalian cell system*
**作者**：Smith A, Johnson B, et al. (2016)
**摘要**：本研究成功在HEK293细胞中表达了重组人MAN2B2蛋白，并通过亲和层析纯化获得高纯度产物。酶活性分析表明，MAN2B2在酸性pH条件下具有α-甘露糖苷酶活性，提示其在溶酶体糖蛋白降解中的潜在作用。
2. **文献名称**：*Structural insights into the catalytic mechanism of MAN2B2 through X-ray crystallography*
**作者**：Zhang Y, Wang L, et al. (2019)
**摘要**：通过X射线晶体学解析了重组人MAN2B2蛋白的三维结构，发现其活性中心含有保守的酸性残基，与底物结合的关键区域可能与MAN2B1相似，但存在独特的构象变化，为进一步研究其功能差异提供了结构基础。
3. **文献名称**：*MAN2B2 overexpression promotes tumor metastasis via glycosylation pathway modulation*
**作者**：Chen X, Lee H, et al. (2021)
**摘要**：研究发现，在多种癌症细胞系中，重组人MAN2B2蛋白的表达上调与N-糖基化修饰异常相关，可能通过调控细胞表面受体(如整合素)的糖基化水平促进肿瘤侵袭和转移。
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**备注**：上述文献为假设示例，实际研究中可能需要通过数据库(如PubMed、Web of Science)检索具体文献。MAN2B2的研究方向主要集中在蛋白结构、酶学特性及与疾病(如癌症、神经退行性疾病)的关联。
Recombinant human MAN2B2 protein is a genetically engineered form of lysosomal alpha-mannosidase, encoded by the MAN2B2 gene located on chromosome 4q25. As a member of the glycosyl hydrolase 38 family, MAN2B2 plays a role in N-linked glycoprotein catabolism by cleaving alpha-1.2-, alpha-1.3-, and alpha-1.6-mannose residues during lysosomal degradation. Unlike its well-studied paralog MAN2B1 (deficient in alpha-mannosidosis), MAN2B2's physiological significance remains less defined, though it may compensate for partial MAN2B1 dysfunction. Recombinant production typically involves expression in mammalian systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications critical for enzymatic activity. This purified protein serves as a vital tool for studying substrate specificity, enzyme kinetics, and potential therapeutic applications. Recent research explores its involvement in cellular processes beyond glycoprotein degradation, including immune regulation and tumor progression. Its recombinant form enables structural studies (e.g., X-ray crystallography) to map catalytic domains and design targeted inhibitors. Challenges persist in fully characterizing its endogenous substrates and tissue-specific functions, driving ongoing investigations into its role in rare lysosomal storage disorders and age-related pathologies.
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