| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAN2A1 |
| Uniprot No | Q16706 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1045-1144aa |
| 活性数据 | DIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFRIQLR |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAN2A1蛋白的3篇参考文献示例(均为模拟内容，供参考)：
1. **文献名称**："Expression and purification of recombinant human MAN2A1 in insect cells"
**作者**：Chen, L., et al.
**摘要**：该研究报道了利用杆状病毒-昆虫细胞系统高效表达重组人MAN2A1蛋白，通过亲和层析纯化获得高活性酶，并验证其切割N-糖链α-1.6-甘露糖苷酶的活性。
2. **文献名称**："Structural insights into MAN2A1's catalytic mechanism by X-ray crystallography"
**作者**：Zhang, Y., et al.
**摘要**：通过X射线晶体学解析了重组人MAN2A1的3D结构，揭示了其底物结合口袋的关键氨基酸残基，为针对癌症的酶活性抑制剂设计提供了结构基础。
3. **文献名称**："MAN2A1 overexpression promotes prostate cancer metastasis via glycosylation modulation"
**作者**：Park, S., et al.
**摘要**：研究利用重组MAN2A1蛋白体外验证其通过调控EGFR蛋白的糖基化修饰增强前列腺癌细胞迁移能力，提示其作为癌症治疗靶点的潜力。
(注：以上文献为示例，实际引用时请核实真实出版物。)
Recombinant human MAN2A1 protein is a genetically engineered form of the alpha-mannosidase 2A1 enzyme, encoded by the *MAN2A1* gene located on chromosome 15q25. This Golgi-resident glycosidase plays a critical role in N-linked glycan processing, specifically trimming mannose residues during the maturation of complex glycoproteins. By catalyzing the hydrolysis of α-1.3 and α-1.6 mannose linkages, MAN2A1 enables the formation of hybrid and complex oligosaccharide structures essential for protein folding, stability, and cellular recognition. Dysregulation of MAN2A1 has been linked to cancer metastasis, immune disorders, and congenital disorders of glycosylation. Recombinant MAN2A1 is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Its applications span structural studies, enzymatic activity assays, and drug discovery research targeting glycosylation-related pathologies. Recent studies also explore its potential as a biomarker in malignancies, as abnormal MAN2A1 expression correlates with altered cell adhesion and tumor progression. Purified recombinant protein allows researchers to investigate substrate specificity, inhibition mechanisms, and therapeutic interventions with precision.
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