纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | LZTS1 |
Uniprot No | P60853 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-599aa |
活性数据 | GSVSSLISGHSFHSKHCRASQYKLRKSSHLKKLNRYSDGLLRFGFSQDSGRGKSSSKMGKSEDFFYIKVSQKARGSHRPDYTALSSGDIGGQTGVDFDPATPPKLMPFSNQLEMSSDKGAVRPTAFKPVLPRSGAILHSSPESTSHQLHPMPPDKPKEQELKPGLCSGALSDSGRNSMSSLPTHSTTSSYQLDPLVTPVGPTSRFGGSAHNITQGIILQDSNMMSLKALSFSDGGSKLAHPGKADKGASCVRSPLSTDECTIQELEQKLLQRETALQKLQRSFDEKEFASGQTFEERPRRTRDELECLEPKSKLKPPSQKSQRTQQVLQLQVLQLQQEKRQLRQELESLMKEQDLLETKLRSYEREKTNFAPALEETQWEVCQKSGEISLLKQQLKESQLEVNTKASEILSLKAQLKDTRGKLDGMELKTQDLESALRTKGLELEVCENELQRKKNEAELLREKVNLLEQELMELRAQAALHPAPLGPPGVGLTFSEDIPALQRELDRLRAELKEERQGHDQMSSGFQHERLVWKEEKEKVIQYQRQLQQSYLAMYQRNQRLEKALQQLARGDGPGEPFEIDLEGADIPYEDIIATEI |
分子量 | 74.1 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性的参考文献示例,供参考:
1. **文献名称**:*Cloning and Functional Characterization of Recombinant Mouse LZTS1 Protein in Cell Cycle Regulation*
**作者**:Ishii H, et al.
**摘要**:该研究成功克隆并表达了重组鼠LZTS1蛋白,证明其通过调节Cyclin D1和p21蛋白表达抑制G1/S期细胞周期转换,提示其作为肿瘤抑制因子的潜在机制。
2. **文献名称**:*Recombinant Murine LZTS1 Inhibits Tumor Cell Migration via Modulating EMT Pathways*
**作者**:Zhang Y, et al.
**摘要**:研究利用大肠杆菌系统表达纯化重组鼠LZTS1.发现其过表达显著降低黑色素瘤细胞迁移能力,并抑制上皮-间质转化(EMT)相关标志物。
3. **文献名称**:*LZTS1 Protein Interacts with DNA Damage Repair Complexes in Mouse Embryonic Fibroblasts*
**作者**:Kagawa S, et al.
**摘要**:通过免疫共沉淀实验,作者证实重组鼠LZTS1与BRCA1和Rad51相互作用,参与调控DNA损伤修复,缺失LZTS1导致基因组稳定性下降。
4. **文献名称**:*Optimization of Recombinant LZTS1 Production in Insect Cells for Structural Studies*
**作者**:Smith TJ, Patel R
**摘要**:研究改进了杆状病毒-昆虫细胞系统中重组鼠LZTS1的表达和纯化工艺,获得高纯度蛋白用于结晶实验,为后续结构解析奠定基础。
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**注**:上述文献为示例,实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
The leucine zipper tumor suppressor 1 (LZTS1), also known as FEZ1. is a protein encoded by the *Lzts1* gene, first identified for its role in tumor suppression. In mice, LZTS1 is highly conserved and shares structural and functional similarities with its human homolog. It contains a leucine zipper domain critical for protein-protein interactions and a C-terminal region implicated in cell cycle regulation. Studies suggest LZTS1 modulates mitotic progression by interacting with microtubules or cell cycle regulators like CDK1. ensuring chromosomal stability and proper cell division. Its loss or downregulation, often through epigenetic silencing, correlates with genomic instability and tumorigenesis in various cancers.
Recombinant murine LZTS1 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and tumor-suppressive mechanisms. Purified recombinant LZTS1 facilitates in vitro assays examining its effects on cell proliferation, apoptosis, and migration. Mouse models with *Lzts1* knockout or overexpression highlight its role in cancer development, drug resistance, and metastasis. Research using recombinant LZTS1 also explores its involvement in DNA damage response and interactions with signaling pathways like Wnt/β-catenin. These studies aim to elucidate LZTS1's therapeutic potential as a biomarker or target for cancer treatment.
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