| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FKBP8 |
| Uniprot No | Q14318 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQ PPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKG QVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYG PQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLA ANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLE HQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTE TALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FKBP8重组蛋白的3篇参考文献示例(注：文献为示例性概括，非真实存在)：
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1. **文献名称**：Recombinant FKBP8 suppresses mitochondrial apoptosis by inhibiting Bax activation
**作者**：Sung YH, et al.
**摘要**：该研究通过在大肠杆菌中重组表达人源FKBP8蛋白，发现其通过抑制促凋亡蛋白Bax的线粒体定位，阻断细胞色素c释放，从而发挥抗凋亡功能。
2. **文献名称**：FKBP8 interacts with Hsp90 via its recombinant TPR domain and regulates client protein stability
**作者**：Edlich F, et al.
**摘要**：利用重组表达的FKBP8-TPR结构域，揭示了其与分子伴侣Hsp90的相互作用机制，并证明这一结合对Hsp90客户蛋白的稳定性至关重要。
3. **文献名称**：FKBP8 recruits LC3A to mitochondria during selective autophagy
**作者**：Kang BH, et al.
**摘要**：通过重组FKBP8蛋白的体外实验，发现其通过LC3A结合域介导自噬小体与线粒体的靶向结合，调控线粒体自噬(mitophagy)进程。
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以上内容基于FKBP8研究的典型方向综合而成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
FKBP8. also known as FK506-binding protein 8 or Prolyl isomerase FKBP8. is a member of the FKBP family of immunophilins that play critical roles in protein folding, signal transduction, and cellular stress responses. It is a unique membrane-associated FKBP protein localized primarily to the mitochondria and endoplasmic reticulum. Structurally, FKBP8 contains an N-terminal transmembrane domain, a FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase) domain, and a C-terminal tetratricopeptide repeat (TPR) domain. Unlike other FKBPs, it lacks calcineurin-inhibitory activity and exhibits selective binding to specific substrates.
Recombinant FKBP8 proteins are engineered to study its molecular functions, particularly its role in regulating apoptosis, autophagy, and mitochondrial dynamics. FKBP8 interacts with Bcl-2 family proteins, such as Bcl-2 and Bcl-xL, to inhibit apoptosis by stabilizing mitochondrial membranes. It also participates in autophagy through its association with mTORC1 and the Unc-51-like kinase 1 (ULK1) complex. Additionally, FKBP8 is implicated in neural development, as knockout models show neural tube defects.
The recombinant protein is produced using bacterial or mammalian expression systems, often tagged for purification and detection. Its applications include elucidating FKBP8’s role in cancer progression, neurodegenerative diseases (e.g., Parkinson’s), and metabolic disorders. Research on FKBP8 also explores its potential as a therapeutic target, given its involvement in stress adaptation pathways. However, its dual pro-survival and context-dependent apoptotic roles necessitate further mechanistic studies to clarify its therapeutic implications.
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