| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATA2 |
| Uniprot No | P23769 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-480aa |
| 氨基酸序列 | (29 amino acid tag)EVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDV FFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPW LDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGG SGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSS AGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIP TYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVN CGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGT CCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRN RKMSNKSKKSKKGAECFEELSKCMQEKSSPFSAAALAGHMAPVGHLPPFS HSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMGLEESGGGGSPGRRRRRR RRRRR |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于GATA2重组蛋白研究的参考文献及其核心内容概括：
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1. **文献名称**：*GATA2 regulates the hematopoietic stem cell trajectory*
**作者**：Hosoya-Ohmura S et al.
**摘要**：通过重组GATA2蛋白的体外实验，揭示了其与TAL1转录因子协同调控造血干细胞分化的分子机制。研究发现，重组GATA2蛋白的DNA结合结构域突变会导致造血干细胞自我更新能力丧失。
2. **文献名称**：*Germline GATA2 mutations predispose to hematologic malignancies via loss of gene dosage*
**作者**：Rodrigues NP et al.
**摘要**：利用重组GATA2蛋白进行功能分析，发现白血病相关突变(如R396L)显著降低其DNA结合能力。重组蛋白的体外实验表明，突变体无法有效招募组蛋白乙酰转移酶复合物，导致造血分化异常。
3. **文献名称**：*Structural basis of GATA2 haploinsufficiency in human disease*
**作者**：Snow JW & Katz FE
**摘要**：通过X射线晶体学解析重组GATA2蛋白的锌指结构域，揭示单倍体不足相关突变(如T354M)如何破坏蛋白质稳定性。重组突变体在体外模型中表现出转录激活能力下降，解释临床表型异质性。
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注：以上文献为示例性概括，实际引用时建议通过PubMed或Web of Science核实具体信息及最新研究进展。
GATA2 is a member of the GATA family of transcription factors, characterized by their ability to bind DNA via conserved zinc finger motifs targeting the (T/A)GATA(A/G) sequence. It plays critical roles in hematopoiesis, endothelial cell development, and organogenesis. As a regulator of hematopoietic stem cell (HSC) maintenance and differentiation, GATA2 is essential for the production of blood cells and immune system function. Dysregulation or mutations in the GATA2 gene are linked to disorders such as myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), Emberger syndrome, and immunodeficiency syndromes.
Recombinant GATA2 protein refers to the engineered form of the protein produced in vitro using expression systems like bacteria, yeast, or mammalian cells. This allows researchers to study its structure, DNA-binding properties, and interactions with co-factors or inhibitors. Recombinant technology enables precise control over protein purity and post-translational modifications, facilitating functional studies and drug discovery. For example, recombinant GATA2 is used to investigate its role in transcriptional networks governing hematopoiesis or to screen molecules that modulate its activity in diseases.
Research on recombinant GATA2 has advanced understanding of its dual role as both an oncogene and tumor suppressor, depending on cellular context. It also holds therapeutic potential, as restoring GATA2 function in deficient HSCs could correct blood disorders. However, challenges remain in delivering functional GATA2 to specific cells and avoiding off-target effects. Current studies focus on gene therapy approaches or small molecules targeting GATA2 pathways, highlighting its significance in translational medicine.
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