| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GABPa |
| Uniprot No | Q06546 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-454aa |
| 氨基酸序列 | MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALATASLQTEKDN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABPa重组蛋白的3篇虚构参考文献示例(基于学术研究常见方向，非真实文献)：
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1. **文献名称**：*Structural and Functional Analysis of Recombinant GABPa in Transcriptional Regulation*
**作者**：Chen L, et al.
**摘要**：本研究通过大肠杆菌表达系统纯化重组GABPa蛋白，结合X射线晶体学解析其DNA结合结构域的结构，揭示了GABPa与靶基因启动子结合的分子机制，并验证其与GABPβ亚基协同调控线粒体相关基因的功能。
2. **文献名称**：*GABPa Recombinant Protein Modulates Cell Cycle Progression in Cancer Cells*
**作者**：Smith JR, et al.
**摘要**：利用重组GABPa蛋白处理多种癌细胞系，发现其通过调控Cyclin D1等基因表达抑制G1/S期转换，并证实GABPa的磷酸化修饰对其功能具有关键影响，为癌症靶向治疗提供了潜在策略。
3. **文献名称**：*In Vitro Reconstitution of GABPa/β Complex and Its Role in Mitochondrial Biogenesis*
**作者**：Wang Y, et al.
**摘要**：通过共表达纯化获得GABPa/β异源二聚体重组蛋白，结合体外荧光素酶报告系统证明其特异性激活NRF-1等线粒体生物合成相关基因，并发现ROS信号可增强该复合体的转录活性。
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注：以上文献为模拟内容，实际研究中建议通过PubMed或Google Scholar以“GABPa recombinant protein”等关键词检索真实文献。
**Background of GABPa Recombinant Protein**
GABPa (GA-binding protein alpha) is a member of the Ets family of transcription factors, which regulate gene expression by binding to specific DNA sequences. It typically forms a heterodimer with GABPb (GABPβ) to activate or repress target genes involved in critical cellular processes, including cell cycle regulation, mitochondrial biogenesis, immune responses, and cellular differentiation. Structurally, GABPa contains an Ets DNA-binding domain that recognizes purine-rich GA-binding motifs and a transactivation domain essential for recruiting co-regulators.
The GABP complex plays a pivotal role in maintaining cellular homeostasis. For example, it regulates nuclear-encoded mitochondrial genes by cooperating with nuclear respiratory factors (NRFs), linking it to energy metabolism and oxidative phosphorylation. Dysregulation of GABPa has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. In cancer, aberrant GABPa activity may drive tumor proliferation or survival, making it a potential therapeutic target.
Recombinant GABPa protein is produced using expression systems like *E. coli* or mammalian cell lines, enabling studies on its structure, DNA-binding properties, and interactions. Purified recombinant GABPa is widely used in *in vitro* assays, including electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and reporter gene analyses, to dissect its regulatory mechanisms. Additionally, engineered mutants of recombinant GABPa help identify functional domains or phosphorylation sites critical for its activity.
The availability of recombinant GABPa has advanced research into its biological roles and therapeutic potential, offering tools for drug screening and mechanistic studies in diseases linked to transcriptional dysregulation. Its application continues to shed light on how transcription factor networks orchestrate complex cellular behaviors.
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