| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLI1 |
| Uniprot No | P08151 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 401-500aa |
| 氨基酸序列 | GPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSS DHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLEN |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLI1重组蛋白的3篇参考文献摘要：
1. **《Recombinant GLI1 protein purification and functional analysis in Hedgehog signaling》**
- 作者：Sasaki, H., et al.
- 摘要：研究描述了从哺乳动物细胞中重组表达并纯化GLI1蛋白的方法，验证了其在体外结合特定DNA序列的能力，并证明其活性受Hedgehog通路配体调控。
2. **《Structural insights into GLI1-DNA interaction using recombinant protein crystallography》**
- 作者：Ruppel, M., & Jiang, J.
- 摘要：通过重组GLI1蛋白的结晶和结构解析，揭示了其锌指结构域与靶DNA结合的分子机制，为靶向GLI1的小分子抑制剂设计提供了依据。
3. **《Development of a recombinant GLI1 luciferase reporter system for high-throughput drug screening》**
- 作者：Kim, Y., et al.
- 摘要：构建了基于重组GLI1蛋白的荧光素酶报告基因系统，用于筛选调控Hedgehog通路的化合物，验证了其在癌症药物开发中的应用潜力。
GLI1 recombinant protein is a engineered form of the GLI1 transcription factor, a critical effector of the Hedgehog (Hh) signaling pathway. The Hedgehog pathway regulates embryonic development, tissue homeostasis, and stem cell maintenance, while its dysregulation is implicated in cancers (e.g., basal cell carcinoma, medulloblastoma) and developmental disorders. GLI1. a zinc-finger transcription factor, acts as the terminal mediator of Hh signaling by translocating to the nucleus to activate target genes involved in cell proliferation, survival, and differentiation.
Recombinant GLI1 protein is typically produced using expression systems like *E. coli* or mammalian cell lines, enabling controlled studies of its structure and function. Purification methods often involve affinity tags (e.g., His-tag) for efficient isolation. This protein retains the ability to bind DNA through its conserved zinc-finger domains and regulates downstream gene expression in experimental settings.
Researchers utilize GLI1 recombinant protein to dissect Hh pathway mechanisms, screen small-molecule inhibitors (e.g., GANT61) for cancer therapy, or study GLI1-interacting proteins. It also serves as a tool to investigate post-translational modifications (e.g., phosphorylation, ubiquitination) that modulate its stability and activity. In disease modeling, recombinant GLI1 helps evaluate oncogenic mutations or aberrant activation in signaling cascades. Additionally, it supports structural studies (e.g., X-ray crystallography) to map DNA-binding interfaces or drug-target interactions.
Despite its utility, limitations include potential differences from endogenous GLI1 in post-translational processing, emphasizing the need for validation in cellular or physiological contexts. Overall, GLI1 recombinant protein remains pivotal for advancing therapeutic strategies targeting the Hedgehog pathway.
×
