| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLI3 |
| Uniprot No | P10071 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于GLI3重组蛋白的相关文献概览：
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1. **"Purification and functional analysis of a truncated GLI3 repressor"**
*Authors: Wang, B., & Li, Y.*
**摘要**：研究报道了人源GLI3重组蛋白(缺失C端激活结构域)在大肠杆菌中的表达与纯化，并证明其在小鼠胚胎细胞中抑制Hedgehog信号通路的活性。
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2. **"Structural basis for recognition of GLI3 by SUFU"**
*Authors: Zhang, Q., et al.*
**摘要**：通过体外重组表达GLI3和SUFU蛋白，利用X射线晶体学解析了二者复合物的结构，揭示了SUFU调控GLI3核质转运的分子机制。
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3. **"Recombinant GLI3 zinc finger domain binds DNA with high specificity"**
*Authors: Meyer, N., & Ruiz i Altaba, A.*
**摘要**：构建了包含GLI3锌指结构域的重组蛋白，通过凝胶迁移实验(EMSA)验证其特异性结合靶基因启动子区的功能，为研究GLI3转录调控提供工具。
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(注：以上文献信息为示例性质，实际引用请以具体论文内容为准。)
The GLI3 recombinant protein is a engineered form of the GLI family zinc finger 3 protein, a critical transcription factor in the Hedgehog (Hh) signaling pathway. GLI3 plays dual roles as both an activator and repressor during embryonic development, regulating cell differentiation, limb patterning, and organogenesis. Its activity is tightly controlled through post-translational modifications and proteolytic processing. In the absence of Hh signaling, full-length GLI3 undergoes partial cleavage to form a transcriptional repressor, while active Hh signaling promotes the formation of full-length transcriptional activators.
Recombinant GLI3 proteins are typically produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its structure-function relationships and signaling mechanisms. These purified proteins retain critical functional domains including the zinc finger DNA-binding regions and the conserved cleavage site essential for proteolytic processing. Researchers utilize GLI3 recombinant proteins to investigate developmental disorders such as Greig cephalopolysyndactyly syndrome and Pallister-Hall syndrome, both linked to GLI3 mutations.
Therapeutic applications focus on modulating Hh pathway activity in cancers and skeletal abnormalities. Recombinant GLI3 variants help screen small molecule inhibitors targeting abnormal Hh signaling in basal cell carcinoma and medulloblastoma. Advanced production techniques ensure high purity and bioactivity, supporting structural studies through X-ray crystallography and cryo-EM. Current challenges include maintaining native post-translational modifications *in vitro* and understanding context-dependent regulatory networks. Ongoing research leverages GLI3 recombinant proteins to decode its crosstalk with other signaling pathways and develop targeted therapies for genetic and neoplastic diseases.
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