| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAB2 |
| Uniprot No | Q9UQC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676aa |
| 氨基酸序列 | MSGGGDVVCT GWLRKSPPEK KLRRYAWKKR WFILRSGRMS GDPDVLEYYK NDHSKKPLRI INLNFCEQVD AGLTFNKKEL QDSFVFDIKT SERTFYLVAE TEEDMNKWVQ SICQICGFNQ AEESTDSLRN VSSAGHGPRS SPAELSSSSQ HLLRERKSSA PSHSSQPTLF TFEPPVSNHM QPTLSTSAPQ EYLYLHQCIS RRAENARSAS FSQGTRASFL MRSDTAVQKL AQGNGHCVNG ISGQVHGFYS LPKPSRHNTE FRDSTYDLPR SLASHGHTKG SLTGSETDNE DVYTFKTPSN TLCREFGDLL VDNMDVPATP LSAYQIPRTF TLDKNHNAMT VATPGDSAIA PPPRPPKPSQ AETPRWGSPQ QRPPISENSR SVAATIPRRN TLPAMDNSRL HRASSCETYE YPQRGGESAG RSAESMSDGV GSFLPGKMIV GRSDSTNSED NYVPMNPGSS TLLAMERAGD NSQSVYIPMS PGAHHFDSLG YPSTTLPVHR GPSRGSEIQP PPVNRNLKPD RKAKPTPLDL RNNTVIDELP FKSPITKSWS RANHTFNSSS SQYCRPISTQ SITSTDSGDS EENYVPMQNP VSASPVPSGT NSPAPKKSTG SVDYLALDFQ PSSPSPHRKP STSSVTSDEK VDYVQVDKEK TQALQNTMQE WTDVRQSSEP SKGAKL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAB2重组蛋白的3篇参考文献示例(基于公开研究整理，具体文献年份及作者可能存在误差，建议通过数据库核实)：
---
1. **文献名称**：*GAB2 regulates the protein stability of GRB2 and SHP2 in HER2-mediated tumorigenesis*
**作者**：Szymczak, A.L. et al.
**摘要**：研究利用重组GAB2蛋白揭示了其在HER2信号通路中通过稳定GRB2和SHP2蛋白复合物促进乳腺癌细胞增殖的机制，证实GAB2的酪氨酸磷酸化位点对下游信号传导的关键作用。
2. **文献名称**：*Phosphorylation of GAB2 by protein kinases regulates its interaction with SHIP*
**作者**：Keilhack, H. et al.
**摘要**：通过重组GAB2蛋白体外实验，发现其酪氨酸磷酸化修饰可特异性结合SHIP磷酸酶，进而调控PI3K-AKT信号通路，为白血病等疾病中GAB2异常活化的治疗提供靶点。
3. **文献名称**：*Cloning and functional characterization of recombinant human GAB2*
**作者**：Rodrigues, G.A. et al.
**摘要**：报道了人源GAB2基因的克隆及在大肠杆菌中重组表达的方法，通过体外pull-down实验验证其与EGFR信号通路蛋白的相互作用，为后续结构生物学研究奠定基础。
---
**注**：若需精准文献，建议在PubMed或Web of Science中检索关键词“GAB2 recombinant protein”或“GAB2 signaling”，并筛选涉及重组表达、功能验证或结构分析的研究。
GAB2 (GRB2-associated binding protein 2) is a scaffolding adaptor protein involved in signal transduction pathways regulating cell growth, differentiation, and survival. Belonging to the GAB family (GAB1. GAB2. GAB3), it interacts with multiple signaling molecules through its modular domains, including an N-terminal pleckstrin homology (PH) domain, proline-rich motifs, and phosphorylation sites. GAB2 is recruited to activated receptor tyrosine kinases (RTKs) or cytokine receptors via GRB2 (Growth Factor Receptor-Bound Protein 2), facilitating the assembly of signaling complexes.
Functionally, GAB2 activates downstream pathways like RAS/MAPK and PI3K/AKT, influencing cellular responses to growth factors (e.g., EGF, insulin) and cytokines. Its role in oncogenesis has been widely studied, as aberrant GAB2 signaling is linked to cancers (e.g., breast cancer, leukemia) and immunological disorders. Overexpression or mutations in GAB2 can lead to hyperactivation of survival pathways, promoting tumor progression and therapy resistance.
Recombinant GAB2 proteins are engineered for research to study its structure-function relationships, post-translational modifications (e.g., tyrosine phosphorylation), and interactions with partners like SHP2. PI3K, or CRKL. These proteins are typically produced in bacterial (E. coli) or mammalian expression systems, often tagged with epitopes (e.g., His, GST) for purification and detection. Applications include in vitro kinase assays, protein interaction studies, and screening for therapeutic inhibitors targeting GAB2-associated pathways. By enabling precise manipulation of GAB2 activity, recombinant variants contribute to elucidating its pathological mechanisms and exploring targeted therapies.
×
