| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAB3 |
| Uniprot No | Q8WWW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-586aa |
| 氨基酸序列 | MSAGDAVCTG WLVKSPPERK LQRYAWRKRW FVLRRGRMSG NPDVLEYYRN KHSSKPIRVI DLSECAVWKH VGPSFVRKEF QNNFVFIVKT TSRTFYLVAK TEQEMQVWVH SISQVCNLGH LEDGADSMES LSYTPSSLQP SSASSLLTAH AASSSLPRDD PNTNAVATEE TRSESELLFL PDYLVLSNCE TGRLHHTSLP TRCDSWSNSD RSLEQASFDD VFVDCLQPLP SSHLVHPSCH GSGAQEVPSS RPQAALIWSR EINGPPRDHL SSSPLLESSL SSTIQVDKNQ GSLPCGAKEL DIMSNTPPPR PPKPSHLSER RQEEWSTHSG SKKPECTLVP RRISLSGLDN MRTWKADVEG QSLRHRDKRL SLNLPCRFSP MYPTASASIE DSYVPMSPQA GASGLGPHCS PDDYIPMNSG SISSPLPELP ANLEPPPVNR DLKPQRKSRP PPLDLRNLSI IREHASLTRT RTVPCSRTSF LSPERNGINS ARFFANPVSR EDEESYIEME EHRTASSLSS GALTWTKKFS LDYLALDFNS ASPAPMQQKL LLSEEQRVDY VQVDEQKTQA LQSTKQEWTD ERQSKV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAB3重组蛋白的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**: *GAB3 regulates macrophage inflammatory responses via PI3K/Akt signaling pathway*
**作者**: Liu Y, et al.
**摘要**: 研究利用重组GAB3蛋白在巨噬细胞中验证其通过PI3K/Akt通路调控炎症因子释放的机制，发现GAB3过表达增强TLR4触发的炎症反应。
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2. **文献名称**: *Structural and functional analysis of GAB3 in B cell receptor signaling*
**作者**: Wang H, et al.
**摘要**: 通过重组GAB3蛋白解析其磷酸化结构域，证明GAB3作为衔接蛋白在BCR信号中招募Syk激酶，促进B细胞增殖和分化。
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3. **文献名称**: *GAB3 modulates HER2-mediated oncogenesis in breast cancer cells*
**作者**: Rodriguez G, et al.
**摘要**: 研究使用重组GAB3蛋白揭示其与HER2受体结合后激活MAPK/ERK通路，促进乳腺癌细胞迁移和耐药性的分子机制。
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**备注**：以上文献信息为示例性内容，实际研究中建议通过PubMed或Web of Science以关键词“GAB3 recombinant protein”或“GAB3 signaling”检索最新实证论文。如需具体文献，可进一步提供研究方向(如疾病模型/信号机制)，以便精准推荐。
**Background of GAB3 Recombinant Protein**
GAB3 (Grb2-associated binder 3) is a member of the Gab family of scaffolding adapter proteins, which play critical roles in signal transduction by integrating and amplifying signals from cell surface receptors, particularly receptor tyrosine kinases (RTKs). Structurally, GAB3 contains conserved domains, including an N-terminal pleckstrin homology (PH) domain for membrane targeting, proline-rich motifs for SH3 domain-mediated interactions, and multiple tyrosine phosphorylation sites that recruit downstream effectors such as phosphatidylinositol 3-kinase (PI3K) and SHP2.
GAB3 is predominantly expressed in hematopoietic cells, neurons, and epithelial tissues. It functions as a downstream effector of various cytokines, growth factors, and immune receptors. For example, GAB3 interacts with Grb2 (Growth factor receptor-bound protein 2) to mediate signaling pathways like PI3K/Akt and Ras/MAPK, which regulate cell survival, proliferation, and differentiation. In immune cells, GAB3 is implicated in FcεRI and TREM2 (Triggering Receptor Expressed on Myeloid Cells 2) signaling, influencing inflammatory responses and phagocytosis.
Dysregulation of GAB3 has been linked to pathological conditions. Overexpression or aberrant activation of GAB3 is observed in cancers, including leukemia and breast cancer, where it promotes oncogenic signaling and metastasis. In neurodegenerative diseases like Alzheimer’s, GAB3 is involved in TREM2-associated microglial function, affecting disease progression.
Recombinant GAB3 protein is engineered for *in vitro* studies to dissect its molecular interactions, post-translational modifications, and pathway crosstalk. It is typically produced in bacterial or mammalian expression systems, often tagged with fusion proteins (e.g., His or GST tags) for purification and detection. Researchers utilize recombinant GAB3 to investigate its role in cellular signaling, screen for therapeutic inhibitors, or study mutations linked to diseases.
Overall, GAB3 serves as a pivotal node in cellular signaling networks, with recombinant forms enabling mechanistic insights into its biological functions and therapeutic potential.
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