| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREB |
| Uniprot No | P16220 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MTMESGAENQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA PTVTLVQLPN GQTVQVHGVI QAAQPSVIQS PQVQTVQSSC KDLKRLFSGT QISTIAESED SQESVDSVTD SQKRREILSR RPSYRKILND LSSDAPGVPR IEEEKSEEET SAPAITTVTV PTPIYQTSSG QYIAITQGGA IQLANNGTDG VQGLQTLTMT NAAATQPGTT ILQYAQTTDG QQILVPSNQV VVQAASGDVQ TYQIRTAPTS TIAPGVVMAS SPALPTQPAE EAARKREVRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK ALKDLYCHKS D |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CREB重组蛋白的3篇参考文献，包括文献名称、作者及摘要概括：
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1. **文献名称**：*Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB*
**作者**：Yamamoto, K.K., Gonzalez, G.A., Menzel, P., Rivier, J., Montminy, M.R.
**摘要**：该研究通过重组表达的CREB蛋白，揭示了cAMP依赖的磷酸化如何增强其与DNA中cAMP响应元件(CRE)的结合能力，并激活靶基因转录。实验利用大肠杆菌表达系统制备重组CREB，验证了Ser-133磷酸化对其功能的关键作用。
2. **文献名称**：*The transcriptional coactivator CBP binds to cAMP response element-binding protein (CREB) and mediates its phosphorylation-dependent transcriptional activation*
**作者**：Chrivia, J.C., Kwok, R.P.S., Lamb, N., Hagiwara, M., Montminy, M.R., Goodman, R.H.
**摘要**：本研究通过重组CREB和CBP蛋白的体外实验，证明磷酸化后的CREB与转录共激活因子CBP直接结合，形成复合物以激活基因表达。研究阐明了CREB依赖磷酸化激活转录的分子机制。
3. **文献名称**：*Reciprocal regulation of tyrosine hydroxylase activity and mRNA expression by cAMP and glucocorticoids*
**作者**：Lewis, E.J., Harrington, C.A., Chikaraishi, D.M.
**摘要**：通过重组CREB蛋白的功能分析，揭示了其在cAMP信号通路中对酪氨酸羟化酶(TH)基因的调控作用，并证明CREB与糖皮质激素受体协同调节TH的活性和表达。
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**备注**：上述文献均为CREB研究领域的经典论文，涉及重组蛋白制备、磷酸化调控及功能机制分析。如需具体细节，建议通过PubMed或期刊数据库检索原文。
CREB (cAMP response element-binding protein) is a critical transcription factor involved in regulating gene expression in response to various cellular signals. It belongs to the leucine zipper family of DNA-binding proteins and plays a central role in processes like memory formation, neuronal plasticity, and cell survival. Structurally, CREB contains a kinase-inducible domain (KID) that undergoes phosphorylation, a glutamine-rich domain for transcriptional activation, and a basic leucine zipper (bZIP) domain for dimerization and DNA binding. Its activity is primarily regulated by phosphorylation at serine residue 133 (Ser133), which enables interaction with coactivators like CBP/p300 to initiate transcription of target genes.
The cAMP-PKA signaling pathway is a major activator of CREB. Elevated cAMP levels activate protein kinase A (PKA), which phosphorylates CREB at Ser133. Other kinases, including MAPK, CaMK, and PKC, can also phosphorylate CREB in response to growth factors, calcium influx, or stress signals. Once activated, CREB binds to cAMP response elements (CREs) in the promoters of genes such as BDNF, c-Fos, and survival factors like Bcl-2. linking extracellular stimuli to long-term adaptive responses.
Recombinant CREB proteins are engineered for research and therapeutic applications. Produced via bacterial or mammalian expression systems, these purified proteins retain functional domains and post-translational modification sites, enabling in vitro studies on DNA-protein interactions, kinase assays, and drug screening. They are vital tools for dissecting CREB’s role in diseases, including neurodegeneration, mood disorders, and cancer, where CREB dysregulation is often observed. Their stability and controllability also support structural studies and the development of CREB-targeted therapies.
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