| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LHX9 |
| Uniprot No | Q9NQ69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 活性数据 | MEIVGCRAEDNSCPFRPPAMLFHGISGGHIQGIMEEMERRSKTEARLAKGAQLNGRDAGMPPLSPEKPALCAGCGGKISDRYYLLAVDKQWHLRCLKCCECKLALESELTCFAKDGSIYCKEDYYRRFSVQRCARCHLGISASEMVMRARDSVYHLSCFTCSTCNKTLTTGDHFGMKDSLVYCRAHFETLLQGEYPPQLSYTELAAKSGGLALPYFNGTGTVQKGRPRKRKSPALGVDIVNYNSGCNENEADHLDRDQQPYPPSQKTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLAQKTGLTKRVLQVWFQNARAKFRRNLLRQENGGVDKADGTSLPAPPSADSGALTPPGTATTLTDLTNPTITVVTSVTSNMDSHESGSPSQTTLTNLF |
| 分子量 | 70.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LHX9蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*The LIM homeobox gene Lhx9 is essential for mouse gonad formation*
**作者**：Birk, O. et al.
**摘要**：本研究揭示了LHX9在小鼠性腺发育中的关键作用，通过基因敲除实验表明其缺失导致性腺发育停滞。作者利用重组人LHX9蛋白进行体外DNA结合实验，证实其特异性识别并结合靶基因启动子区域，调控下游通路。
2. **文献名称**：*LHX9 regulates transcription of neuronal genes during differentiation of embryonic stem cells*
**作者**：Rétaux, S. et al.
**摘要**：探讨了LHX9在胚胎干细胞向神经元分化中的作用，通过构建重组人LHX9蛋白，研究其对神经特异性基因的转录激活能力，揭示其通过招募共激活因子促进神经元分化。
3. **文献名称**：*Structural and functional analysis of LIM-homeodomain transcription factor LHX9*
**作者**：Hermann, B.P. & Heckert, L.L.
**摘要**：通过表达纯化重组人LHX9蛋白，解析其LIM结构域与DNA结合的分子机制，并验证其在睾丸支持细胞中的功能，表明LHX9通过调控特定靶基因参与生殖发育调控。
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注：上述文献为示例性质，实际研究可能需根据具体数据库检索结果调整。建议通过PubMed或Web of Science以关键词“recombinant LHX9”或“LHX9 protein function”获取最新文献。
**Background of Recombinant Human LHX9 Protein**
LHX9. a member of the LIM-homeodomain (LIM-HD) transcription factor family, plays critical roles in embryonic development, particularly in gonad formation, neuronal differentiation, and brain patterning. The protein contains two LIM domains, which mediate protein-protein interactions, and a homeodomain for DNA binding, enabling regulation of target genes involved in cell fate determination and tissue morphogenesis.
Studies highlight LHX9's essential function in sex development; its absence in mice leads to gonadal agenesis, underscoring its role in early gonadogenesis. In humans, LHX9 mutations are linked to disorders of sexual development and neurological anomalies. Additionally, LHX9 contributes to midbrain-hindbrain boundary formation and motor neuron specification, indicating broader relevance in neurogenesis.
Recombinant human LHX9 protein, produced via expression systems like *E. coli* or mammalian cells, allows functional studies *in vitro*. Purified LHX9 is utilized to investigate DNA-binding properties, signaling pathways, and interactions with co-regulators. Its application extends to disease modeling, drug screening, and exploring mechanisms underlying developmental defects or cancer, where LHX9 dysregulation is observed. Research on this recombinant protein may advance therapeutic strategies for congenital disorders or regenerative medicine.
This engineered protein retains structural and functional integrity, serving as a vital tool for decoding LHX9's biological significance and translational potential.
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