| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LHX4 |
| Uniprot No | Q969G2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 活性数据 | MMQSATVPAE GAVKGLPEML GVPMQQIPQC AGCNQHILDK FILKVLDRHW HSSCLKCADC QMQLADRCFS RAGSVYCKED FFKRFGTKCT ACQQGIPPTQ VVRKAQDFVY HLHCFACIIC NRQLATGDEF YLMEDGRLVC KEDYETAKQN DDSEAGAKRP RTTITAKQLE TLKNAYKNSP KPARHVREQL SSETGLDMRV VQVWFQNRRA KEKRLKKDAG RHRWGQFYKS VKRSRGSSKQ EKESSAEDCG VSDSELSFRE DQILSELGHT NRIYGNVGDV TGGQLMNGSF SMDGTGQSYQ DLRDGSPYGI PQSPSSISSL PSHAPLLNGL DYTVDSNLGI IAHAGQGVSQ TLRAMAGGPT SDISTGSSVG YPDFPTSPGS WLDEMDHPPF |
| 分子量 | 43.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LHX4蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *"Functional characterization of recombinant human LHX4 protein in pituitary development"*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统表达重组人LHX4蛋白，验证其与下游基因启动子的结合能力，并证明其在体外模型中促进垂体前叶细胞分化的作用。
2. **文献名称**: *"Structural insights into the DNA-binding domains of LHX4 and their role in transcriptional regulation"*
**作者**: Chen L, et al.
**摘要**: 利用重组LHX4蛋白的晶体结构分析，揭示其LIM结构域与特定DNA基序的互作机制，为理解LHX4在神经发育中的调控提供结构基础。
3. **文献名称**: *"LHX4 mutations disrupt protein function: Rescue by recombinant wild-type LHX4 in cellular models"*
**作者**: Wang Y, et al.
**摘要**: 在携带LHX4突变的细胞模型中，外源性重组LHX4蛋白恢复了靶基因的转录活性，支持其潜在治疗先天性垂体缺陷的可行性。
4. **文献名称**: *"Production and purification of bioactive recombinant human LHX4 for functional assays"*
**作者**: Gonzalez R, et al.
**摘要**: 优化哺乳动物细胞表达体系生产高纯度LHX4蛋白，并通过体外实验证实其调控促甲状腺激素释放激素(TRH)的能力。
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**提示**：实际文献需通过学术数据库(如PubMed、Google Scholar)检索关键词“recombinant LHX4 protein”“LHX4 gene function”等获取。
Recombinant human LHX4 protein is a genetically engineered form of the LHX4 transcription factor, which belongs to the LIM-homeodomain (LIM-HD) family. LHX4 plays critical roles in embryonic development, particularly in the formation of the pituitary gland, central nervous system, and respiratory control pathways. It contains two N-terminal LIM domains, which mediate protein-protein interactions, and a homeodomain that binds DNA to regulate gene expression. Mutations in the LHX4 gene are linked to congenital pituitary hormone deficiencies and structural brain abnormalities, highlighting its importance in neuroendocrine development.
Produced using recombinant DNA technology in systems like Escherichia coli or mammalian cell cultures, the purified protein retains functional domains necessary for research applications. Studies employ recombinant LHX4 to investigate its mechanisms in cell differentiation, tissue patterning, and transcriptional regulation. It also serves as a tool to model developmental disorders, screen therapeutic targets, or explore gene therapy strategies. For instance, LHX4 dysfunction is associated with combined pituitary hormone deficiency (CPHD), leading to growth impairments and metabolic issues. By analyzing recombinant LHX4 interactions with co-factors like PITX1 or transcriptional targets such as PROP1. researchers aim to unravel pathogenic pathways and potential interventions. Its production enables standardized in vitro assays, overcoming challenges in isolating native LHX4 from tissues. Ongoing research continues to expand its utility in developmental biology and regenerative medicine.
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