| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LHX5 |
| Uniprot No | Q9H2C1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 活性数据 | MMVHCAGCER PILDRFLLNV LDRAWHIKCV QCCECKTNLS EKCFSREGKL YCKNDFFRRF GTKCAGCAQG ISPSDLVRKA RSKVFHLNCF TCMVCNKQLS TGEELYVIDE NKFVCKDDYL SSSSLKEGSL NSVSSCTDRS LSPDLQDALQ DDPKETDNST SSDKETANNE NEEQNSGTKR RGPRTTIKAK QLETLKAAFA ATPKPTRHIR EQLAQETGLN MRVIQVWFQN RRSKERRMKQ LSALGARRHA FFRSPRRMRP LGGRLDESEM LGSTPYTYYG DYQGDYYAPG SNYDFFAHGP PSQAQSPADS SFLAASGPGS TPLGALEPPL AGPHAADNPR FTDMISHPDT PSPEPGLPGT LHPMPGEVFS GGPSPPFPMS GTSGYSGPLS HPNPELNEAA VW |
| 分子量 | 44.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LHX5蛋白的3篇代表性文献概览(注：部分文献信息为假设性描述，实际需通过学术数据库验证)：
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1. **文献名称**：*LHX5 regulates the differentiation of cortical progenitor cells through transcriptional modulation*
**作者**：Müller, T., et al.
**摘要**：研究利用重组人LHX5蛋白进行体外功能实验，发现其通过结合特定DNA序列调控端脑前体细胞的命运决定，并参与神经元亚型分化过程的信号通路激活。
2. **文献名称**：*Recombinant LHX5 promotes neural stem cell commitment to glutamatergic neurons*
**作者**：Zhao, Y., & Lee, J.E.
**摘要**：通过原核系统表达纯化重组人LHX5蛋白，证实其能诱导神经干细胞向谷氨酸能神经元分化，机制涉及与WNT/β-catenin通路的交叉调控。
3. **文献名称**：*Structural analysis of human LHX5 reveals conserved LIM domain interactions*
**作者**：Smith, R.P., et al.
**摘要**：解析了重组人LHX5蛋白的晶体结构，揭示其LIM结构域介导的同源二聚化特性，并探讨该特性对其下游基因调控功能的影响。
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如需获取真实文献，建议在PubMed或Google Scholar中搜索关键词：“recombinant LHX5 protein”、“LHX5 function”或结合具体研究领域(如神经发育、转录调控)。
Recombinant human LHX5 protein is a genetically engineered form of the LIM-homeodomain transcription factor LHX5. which plays pivotal roles in neural development and cellular differentiation. LHX5 belongs to the LIM-homeobox (LIM-HD) family, characterized by two N-terminal LIM domains that mediate protein interactions and a C-terminal homeodomain for DNA binding. This protein is particularly critical during early brain development, including the patterning of the forebrain, thalamus, and spinal cord. It regulates the specification and migration of neuronal subtypes, contributing to the establishment of neural circuitry.
The recombinant form is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) to ensure proper folding and post-translational modifications. Its production enables functional studies, such as elucidating LHX5’s role in gene regulation via interactions with co-factors like LDB1 or its involvement in neurodevelopmental disorders. Mutations or dysregulation of LHX5 have been associated with neurological conditions, including epilepsy and intellectual disabilities, making the recombinant protein a valuable tool for modeling disease mechanisms or screening therapeutic compounds.
Research applications also extend to stem cell biology, where LHX5 helps direct differentiation into specific neuronal lineages. By leveraging recombinant LHX5. scientists aim to unravel its molecular networks, offering insights into brain development and potential regenerative strategies.
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