| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDB2 |
| Uniprot No | O43679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 活性数据 | MSSTPHDPFYSSPFGPFYRRHTPYMVQPEYRIYEMNKRLQSRTEDSDNLWWDAFATEFFEDDATLTLSFCLEDGPKRYTIGRTLIPRYFSTVFEGGVTDLYYILKHSKESYHNSSITVDCDQCTMVTQHGKPMFTKVCTEGRLILEFTFDDLMRIKTWHFTIRQYRELVPRSILAMHAQDPQVLDQLSKNITRMGLTNFTLNYLRLCVILEPMQELMSRHKTYNLSPRDCLKTCLFQKWQRMVAPPAEPTRQPTTKRRKRKNSTSSTSNSSAGNNANSTGSKKKTTAANLSLSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQYDAANGMDDEEDFNNSPALGNNSPWNSKPPATQETKSENPPPQASQ |
| 分子量 | 66.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LDB2蛋白的3篇示例文献，内容基于常见研究主题，但请注意文献为虚构示例，建议通过学术数据库核实真实文献：
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1. **文献名称**：*Expression and functional analysis of recombinant human LDB2 in vitro*
**作者**：Chen, X., Wang, Y., & Zhang, L.
**摘要**：研究利用昆虫杆状病毒系统表达重组人LDB2蛋白，并验证其与LIM结构域蛋白的结合能力，揭示其在调控细胞分化中的潜在功能。
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2. **文献名称**：*LDB2 Recombinant Protein Enhances Cardiomyocyte Differentiation via Transcriptional Activation*
**作者**：Tanaka, K., et al.
**摘要**：通过在大肠杆菌中重组表达人LDB2蛋白，发现其可促进小鼠胚胎干细胞向心肌细胞分化，并激活心脏特异性基因(如Nkx2.5)的表达。
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3. **文献名称**：*Structural insights into LDB2-mediated transcriptional complexes*
**作者**：Smith, J., et al.
**摘要**：结合X射线晶体学与重组LDB2蛋白，解析其与转录因子GATA4的相互作用结构域，阐明其在心脏发育中的协同调控机制。
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**备注**：上述文献为示例，实际研究需参考真实发表的论文。推荐通过PubMed或Google Scholar搜索关键词(如“LDB2 recombinant protein”、“LDB2 function”)获取权威信息。
LDB2 (LIM-domain-binding protein 2) is a crucial transcriptional regulator in humans, primarily known for its role in mediating protein-protein interactions via its LIM domain-binding module. It belongs to the LDB protein family, which partners with LIM-homeodomain (LIM-HD) transcription factors or LIM-only (LMO) adaptors to form multi-protein complexes. These complexes regulate gene expression programs essential for embryonic development, cell differentiation, and tissue-specific functions. LDB2 is particularly implicated in cardiovascular and neuronal development, with studies linking its dysfunction to congenital heart defects, neurodevelopmental disorders, and cancer progression.
Recombinant human LDB2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interaction networks, and regulatory mechanisms. Its modular structure—featuring a conserved N-terminal dimerization domain and a C-terminal LIM interaction domain—enables scaffolding functions, coordinating transcriptional activation or repression. Researchers utilize recombinant LDB2 to dissect pathways in organogenesis, cellular identity, and disease models. Its role in angiogenesis and synapse formation highlights therapeutic potential, driving interest in structural analyses, CRISPR-based screens, and drug discovery targeting LDB2-dependent oncogenic or developmental pathways. This tool accelerates mechanistic insights into developmental biology and pathology.
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