| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE5A |
| Uniprot No | Q5TCM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-118aa |
| 活性数据 | MSCQQSQQQCQPPPKCTPKCPPKCTPKCPPKCPPKCPPQCSAPCPPPVSSCCGSSSGGCCSSEGGGCCLSHHRPRQSLRRRPQSSSCCGSGSGQQSGGSSCCHSSGGSGCCHSSGGCC |
| 分子量 | 39.38 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LCE5A蛋白的3-4篇参考文献示例，格式为文献名称、作者和简化的摘要内容：
---
1. **《LCE5A作为表皮分化调节因子的功能与表达研究》**
**作者：** Zeeuwen, P.L. 等(2012)
**摘要：** 分析人LCE5A蛋白在表皮分化中的作用，发现其在皮肤屏障形成中具有关键功能，且表达异常与银屑病等炎性皮肤病相关，重组LCE5A可部分修复体外模型中的屏障缺陷。
2. **《银屑病皮肤组织中LCE5A的转录组学下调及其临床意义》**
**作者：** Hänzelmann, S. 等(2015)
**摘要：** 通过RNA-seq技术发现银屑病患者的LCE5A基因显著低表达，可能破坏角质形成细胞的完整性，提示其在疾病中的潜在治疗靶点价值。
3. **《重组人LCE5A蛋白的制备及其抗菌活性研究》**
**作者：** Huang, Y. 等(2018)
**摘要：** 报道利用大肠杆菌系统高效表达重组LCE5A蛋白，证实其具有广谱抗菌活性，尤其在皮肤感染模型中显示出促进组织修复的能力。
4. **《LCE5A基因多态性与自身免疫性皮肤病关联研究》**
**作者：** Chen, J. 等(2017)
**摘要：** 通过GWAS分析揭示LCE5A基因特定SNP位点与银屑病及湿疹易感性相关，重组蛋白功能实验表明部分突变体可能削弱其屏障保护功能。
---
*注：以上文献信息基于学术研究典型方向整合而成，若需真实文献请通过学术数据库查询。*
Recombinant human LCE5A (Late Cornified Envelope 5A) protein is a genetically engineered variant of the naturally occurring LCE5A protein, which belongs to the large family of late cornified envelope (LCE) proteins. These proteins are critical components of the epidermal differentiation complex (EDC) on chromosome 1q21. playing essential roles in skin barrier formation, keratinocyte differentiation, and response to environmental stressors. LCE5A is specifically expressed in the epidermis and is involved in the final stages of cornification, a process where keratinocytes form a protective cornified envelope to maintain skin integrity and hydration.
Structurally, LCE5A shares conserved features with other LCE proteins, including a central domain rich in proline and glycine residues, which may contribute to its cross-linking properties during epidermal maturation. Its expression is regulated by inflammatory cytokines and pathogenic stimuli, linking it to skin disorders such as psoriasis, atopic dermatitis, and ichthyosis. Genetic studies have identified associations between LCE gene cluster deletions (including LCE5A) and susceptibility to psoriasis, highlighting its pathophysiological relevance.
The recombinant form of LCE5A is typically produced in bacterial or mammalian expression systems, enabling researchers to study its biochemical properties, interactions with skin microbiota, and role in inflammatory pathways. Its applications span in vitro disease modeling, drug screening for dermatological therapies, and elucidating mechanisms of skin barrier dysfunction. Ongoing research aims to explore its potential as a therapeutic target or biomarker in chronic inflammatory skin diseases.
×
