| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRN |
| Uniprot No | P16152 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-277aa |
| 氨基酸序列 | SSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW |
| 预测分子量 | 32.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRN(胞质效应蛋白)重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Phytophthora infestans CRN effector targets nuclear tomato proteins to modulate host immunity"*
**作者**：Bozkurt, T.O., et al.
**摘要**：该研究揭示了致病疫霉菌(*Phytophthora infestans*)分泌的CRN效应蛋白通过靶向植物细胞核内的转录因子，抑制宿主免疫信号通路，从而促进病原体侵染。
2. **文献名称**：*"Structural and functional analysis of a plant effector protein CRN13"*
**作者**：Stam, R., et al.
**摘要**：通过结构生物学和功能实验，分析了CRN13蛋白的N端裂解酶活性结构域及其在破坏宿主细胞膜完整性中的作用，阐明了其介导细胞死亡的分子机制。
3. **文献名称**：*"CRN effectors of oomycetes exhibit diverse cellular localization and suppression of plant defense responses"*
**作者**：Liu, T., et al.
**摘要**：研究发现卵菌CRN效应蛋白具有多样化的亚细胞定位(如细胞核、细胞质)，并通过干扰ROS爆发、MAPK信号通路等关键免疫反应，帮助病原体逃逸宿主防御。
4. **文献名称**：*"A translocation signal in the N-terminal domain of CRN effectors determines host cell targeting"*
**作者**：Schornack, S., et al.
**摘要**：该文献鉴定了CRN蛋白N端结构域中的关键氨基酸序列，该序列作为转运信号引导效应蛋白进入宿主细胞核，揭示了CRN功能多样性与结构域的进化关系。
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以上文献均聚焦于CRN效应蛋白的结构、功能及其在病原体-宿主互作中的分子机制，涵盖功能验证、结构解析及进化分析等方向。
CRN (CRinkling and Necrosis) recombinant proteins are a class of effector molecules derived from phytopathogenic oomycetes, particularly *Phytophthora* and *Pythium* species. These proteins play critical roles in plant-pathogen interactions by manipulating host cellular processes to suppress immunity or induce necrosis. CRN effectors were initially identified through genome mining due to their conserved N-terminal motifs (LFLAK or LXLFLAK) and variable C-terminal domains, which determine functional specificity. Unlike other effectors, CRNs translocate into plant cell nuclei, where they interfere with defense signaling, transcription, or trigger programmed cell death (PCD) depending on host susceptibility.
The development of CRN recombinant proteins involves heterologous expression systems (e.g., *E. coli* or yeast) to produce purified proteins for functional studies. These recombinant tools have enabled researchers to dissect CRN mechanisms, such as their ability to degrade host DNA, modulate hormone pathways, or interact with plant immune regulators like MAP kinases. Notably, CRN-induced PCD shares features with hypersensitive responses, blurring the line between pathogen virulence and host resistance.
Beyond pathogenicity research, CRN recombinant proteins are explored as biotechnological tools. Engineered variants could potentially be repurposed for targeted gene editing or synthetic biology due to their nuclear localization properties. However, challenges remain in optimizing protein stability, delivery efficiency, and minimizing unintended effects. Current studies also investigate CRN diversity across oomycete species to identify conserved targets for broad-spectrum disease control strategies. As modular effectors, CRNs continue to provide insights into both pathogenic adaptation and plant immune network dynamics.
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