| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT12 |
| Uniprot No | Q99456 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 活性数据 | MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSASSVGSGYGGSAFGFGASCGGGFSAASMFGSSSGFGGGSGSSMAGGLGAGYGRALGGGSFGGLGMGFGGSPGGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYLDKVRALEEANTELENKIREWYETRGTGTADASQSDYSKYYPLIEDLRNKIISASIGNAQLLLQIDNARLAAEDFRMKYENELALRQGVEADINGLRRVLDELTLTRTDLEMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM |
| 分子量 | 80.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人KRT12蛋白的参考文献示例(部分为模拟概括，仅供参考)：
1. **"Expression and characterization of recombinant human keratin 12 in mammalian cell lines"**
- **作者**: Zhang Y, et al.
- **摘要**: 该研究通过哺乳动物表达系统成功表达重组人KRT12蛋白，并验证其与角膜上皮特异性标记物的相互作用，为研究KRT12在维持角膜透明性中的作用提供了纯化蛋白工具。
2. **"Structural analysis of recombinant KRT12 reveals key domains for corneal epithelial integrity"**
- **作者**: Lee SM, et al.
- **摘要**: 通过X射线晶体学解析重组KRT12蛋白的三维结构，发现其C末端结构域对维持细胞骨架稳定性至关重要，并揭示了与Meesmann角膜营养不良相关的致病突变位点。
3. **"Functional validation of KRT12 mutations using recombinant protein models"**
- **作者**: Chen H, et al.
- **摘要**: 构建携带不同突变体的重组KRT12蛋白，证明特定突变(如p.Arg135Thr)导致蛋白聚合异常，从而破坏角膜上皮细胞屏障功能，为遗传性角膜疾病的机制提供了分子证据。
4. **"Recombinant KRT12 as a therapeutic agent in corneal injury repair"**
- **作者**: Wang X, et al.
- **摘要**: 在体外实验中，重组人KRT12蛋白被证明能促进角膜上皮细胞迁移和创伤愈合，提示其在角膜损伤修复中的潜在治疗价值。
(注：以上文献为概括性示例，实际引用需以真实发表的论文为准。)
Keratin 12 (KRT12) is a cytoskeletal protein belonging to the type I keratin family, which forms intermediate filaments essential for maintaining structural integrity in epithelial cells. Specifically expressed in corneal epithelial cells, KRT12 pairs with KRT3 (type II keratin) to create a protective meshwork that confers mechanical resilience and safeguards against external stressors. Mutations in the KRT12 gene are linked to Meesmann epithelial corneal dystrophy (MECD), a rare inherited disorder characterized by fragile corneal epithelium, microcysts, and photophobia. This highlights KRT12's critical role in corneal homeostasis.
Recombinant human KRT12 protein is engineered using heterologous expression systems (e.g., bacterial, yeast, or mammalian cells) to produce purified, functional KRT12 for research. Its production enables mechanistic studies of KRT12’s interactions with binding partners, its role in cellular stress response, and pathophysiological effects of disease-associated mutations. Researchers use recombinant KRT12 to model corneal dystrophies in vitro, screen therapeutic compounds, and explore gene therapy strategies. Additionally, it aids in developing antibodies for diagnostic applications. Challenges in production include ensuring proper post-translational modifications and solubility, as keratins require partner proteins for stable filament assembly. Despite these hurdles, recombinant KRT12 remains a vital tool for advancing understanding of corneal diseases and therapeutic innovation.
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