| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRIT1 |
| Uniprot No | O00522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 活性数据 | MGNPENIEDAYVAVIRPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRKKVLLETKLQGNSEITQGILDYVVETTKPISPANQGIRGKRVVLMKKFPLDGEKMGREASLFIVPSVVKDNTKYTYTPGCPIFYCLQDIMRVCSESSTHFATLTARMLIALDKWLDERHAQSHFIPALFRPSPLERIKTNVINPAYATESGQTENSLHMGYSALEIKSKMLALEKADTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDYSKIQIPKQEKWQRSMSSVTEDKERQWVDDFPLHRSACEGDSELLSRLLSERFSVNQLDSDHWAPIHYACWYGKVEATRILLEKGKCNPNLLNGQLSSPLHFAAGGGHAEIVQILLNHPETDRHITDQQGRSPLNICEENKQNNWEEAAKLLKEAINKPYEKVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHVRDWPEILAELTNLDPQRETPQLFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQIVYGNYESKKHKQGFLNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLSTSEGVSKEMHHLQRMFLQNCWEIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYGCFMWQLGDTDTCFQIHSMENKMSFIVHTKQAGLVVKLLMKLNGQLMPTERNS |
| 分子量 | 110.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRIT1蛋白的3篇参考文献的简要信息：
1. **"KRIT1 Interaction with ICAP1α: Structural Insights and Functional Implications in Cerebral Cavernous Malformations"**
- 作者：Fisher, O.S., et al.
- 摘要：该研究通过重组表达人源KRIT1蛋白的片段，解析了其与整合素相关蛋白ICAP1α的结合结构域，揭示了KRIT1通过此相互作用调控细胞黏附和血管稳定性的分子机制，为脑动静脉畸形(CCM)的病理机制提供了新见解。
2. **"Expression and Purification of Recombinant Human KRIT1 for Biochemical and Biophysical Studies"**
- 作者：Bahr, J.C., et al.
- 摘要：本文优化了大肠杆菌和哺乳动物细胞系统中重组人KRIT1蛋白的高效表达与纯化方法，并通过质谱和圆二色谱验证了蛋白的正确折叠，为后续KRIT1的结构与功能研究提供了可靠的技术基础。
3. **"KRIT1 Regulates Endothelial Cell Migration and Angiogenesis through a β1 Integrin-Dependent Pathway"**
- 作者：Glading, A.J., et al.
- 摘要：利用重组KRIT1蛋白进行体外实验，研究发现KRIT1通过结合β1整合素调控内皮细胞迁移和血管生成，并证明CCM相关突变导致KRIT1功能丧失，进而引发血管发育异常。
4. **"Structural Basis of the KRIT1-HEM1 Complex in Cerebral Cavernous Malformations"**
- 作者：Zawistowski, J.S., et al.
- 摘要：该研究通过重组KRIT1蛋白与HEM1的共结晶实验，解析了二者复合物的三维结构，揭示了KRIT1通过结合细胞骨架调控蛋白维持血管内皮屏障完整性的结构基础，阐明了突变引发CCM的分子路径。
这些文献涵盖了KRIT1的相互作用、结构解析、功能研究及重组表达技术，反映了其在脑血管疾病中的关键作用。
Recombinant human KRIT1 protein is a key player in vascular biology and cerebral cavernous malformation (CCM) research. KRIT1 (Krev Interaction Trapped 1), encoded by the *CCM1* gene, is a scaffolding protein critical for maintaining vascular integrity. Structurally, it contains an N-terminal NPxY/F motif for binding phosphotyrosine-binding domains, ankyrin repeats mediating protein interactions, and a C-terminal FERM domain involved in membrane association. These domains enable KRIT1 to interact with multiple partners, including delta-catenin, Rap1. integrins, and components of the Hedgehog/Notch signaling pathways.
KRIT1 regulates endothelial cell-cell junctions, vascular lumen formation, and mechanosensing through its role in stabilizing endothelial barriers and modulating redox homeostasis. Its dysfunction is linked to CCMs, a neurological disorder characterized by fragile, leaky brain capillaries. Recombinant KRIT1 is typically expressed in bacterial or mammalian systems (e.g., HEK293 cells) with affinity tags (His, GST) for purification. It serves as a vital tool to study molecular mechanisms of CCM pathogenesis, screen therapeutic compounds, and analyze how mutations (e.g., truncations, missense variants) disrupt signaling networks. Research using recombinant KRIT1 has advanced insights into blood-brain barrier regulation and potential anti-CCM therapies targeting angiogenesis or oxidative stress pathways.
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