| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRI1 |
| Uniprot No | Q8N9T8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-709aa |
| 活性数据 | MGHRTAMPEPRGSSQLRVNAAFAARYNRYREREELQRLKDRYGDRDSSSDSSSESDSSDERVEFDPQQERDFYKTLSLLKKKDPRIYQKDATFYNRTASSSDSEEDPEALEKQKKVRPMYLKDYERKVILEKAGKYVDEENSDGETSNHRLQETSSQSYVEEQKQLKESFRAFVEDSEDEDGAGEGGSSLLQKRAKTRQEKAQEEADYIEWLKGQKEIRNPDSLKELTHLKEYWNDPELDEGERFLRDYILNKRYEEEEEEEEDEEEMEEEEGVHGPPVQLAVDDSSDEGELFLKKQEDFEQKYNFRFEEPDSASVKTYPRSIASSVRRKDERRKEKREETRERKKREKAKKQEELKQLKNLKRKEILAKLEKLRKVTGNEMLGLEEGDLEDDFDPAQHDQLMQKCFGDEYYGAVEEEKPQFEEEEGLEDDWNWDTWDGPEQEGDWSQQELHCEDPNFNMDADYDPSQPRKKKREAPLTGKKKRKSPFAAAVGQEKPVFEPGDKTFEEYLDEYYRLDYEDIIDDLPCRFKYRTVVPCDFGLSTEEILAADDKELNRWCSLKKTCMYRSEQEELRDKRAYSQKAQNSWKKRQVFKSLCREEAETPAEATGKPQRDEAGPQRQLPALDGSLMGPESPPAQEEEAPVSPHKKPAPQKRRRAKKARLLGPTVMLGGCEFSRQRLQAFGLNPKRLHFRQLGRQRRKQQGPKNSS |
| 分子量 | 78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRI1蛋白的示例文献概括(注：KRI1相关研究较少，以下内容为模拟示例，实际文献需通过学术数据库确认)：
1. **文献名称**：Functional Characterization of Recombinant Human KRI1 in Ribosome Biogenesis
**作者**：Zhang L, et al.
**摘要**：该研究通过在大肠杆菌中表达重组人KRI1蛋白，发现其与核糖体RNA加工密切相关，KRI1通过结合pre-rRNA复合体调控核糖体亚基的成熟，缺陷可能导致细胞周期停滞。
2. **文献名称**：Crystal Structure of Human KRI1 Reveals a Novel RNA-Binding Domain
**作者**：Tanaka R, et al.
**摘要**：作者解析了重组KRI1蛋白的晶体结构，发现其含有一个保守的RNA结合基序，并通过体外实验证明其对特定RNA序列的高亲和力，提示其在转录后调控中的作用。
3. **文献名称**：KRI1 Deficiency Disrupts Telomere Maintenance via Impaired rRNA Processing
**作者**：Wang Y, et al.
**摘要**：研究利用重组KRI1蛋白进行功能挽救实验，发现KRI1通过调控rRNA加工间接影响端粒酶活性，缺失导致细胞衰老加速，提示其在衰老相关疾病中的潜在作用。
4. **文献名称**：Development of a KRI1 Knockout Cell Model and Rescue by Recombinant Protein
**作者**：Chen H, et al.
**摘要**：通过CRISPR技术构建KRI1敲除细胞，发现其增殖能力下降;外源性添加纯化的重组人KRI1蛋白可部分恢复细胞表型，表明KRI1在维持基因组稳定性中的功能。
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**备注**：KRI1(又称Homolog of yeast KRI1)在人类中功能研究较少，实际文献可能聚焦于其酵母同源物的机制(如核糖体生成)，建议通过UniProt(ID: Q9BQI3)查询最新研究进展。如需具体文献，可补充基因别名(如C12orf66)进一步检索。
KRI1 (KRI1 homolog, also known as C12orf11) is a conserved eukaryotic protein implicated in ribosome biogenesis and cell cycle regulation. It was initially identified in yeast as a critical factor for 18S rRNA maturation and small ribosomal subunit assembly. Human KRI1. encoded by the *C12orf11* gene on chromosome 12. shares structural and functional homology with its yeast counterpart, featuring WD40 repeat domains that facilitate protein-protein interactions. Studies suggest KRI1 associates with UTP18 and other components of the ribosomal small subunit (SSU) processome, aiding in rRNA processing and ribosome assembly. Dysregulation of KRI1 has been linked to developmental disorders and cancer progression, likely due to its role in maintaining proteostasis and cell proliferation. Recombinant human KRI1 protein (rKRI1) is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, structural analysis, and screening for potential therapeutics targeting ribosomopathies or malignancies. Its applications include investigating ribosome biosynthesis mechanisms, validating protein interaction networks, and serving as an antigen for antibody development. Research on rKRI1 continues to clarify its molecular interactions and regulatory pathways in both normal physiology and disease contexts.
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