| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRTC3 |
| Uniprot No | Q6UUV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 氨基酸序列 | MAASPGSGSA NPRKFSEKIA LHTQRQAEET RAFEQLMTDL TLSRVQFQKL QQLRLTQYHG GSLPNVSQLR SSASEFQPSF HQADNVRGTR HHGLVERPSR NRFHPLHRRS GDKPGRQFDG SAFGANYSSQ PLDESWPRQQ PPWKDEKHPG FRLTSALNRT NSDSALHTSA LSTKPQDPYG GGGQSAWPAP YMGFCDGENN GHGEVASFPG PLKEENLLNV PKPLPKQLWE TKEIQSLSGR PRSCDVGGGN AFPHNGQNLG LSPFLGTLNT GGSLPDLTNL HYSTPLPASL DTTDHHFGSM SVGNSVNNIP AAMTHLGIRS SSGLQSSRSN PSIQATLNKT VLSSSLNNHP QTSVPNASAL HPSLRLFSLS NPSLSTTNLS GPSRRRQPPV SPLTLSPGPE AHQGFSRQLS STSPLAPYPT SQMVSSDRSQ LSFLPTEAQA QVSPPPPYPA PQELTQPLLQ QPRAPEAPAQ QPQAASSLPQ SDFQLLPAQG SSLTNFFPDV GFDQQSMRPG PAFPQQVPLV QQGSRELQDS FHLRPSPYSN CGSLPNTILP EDSSTSLFKD LNSALAGLPE VSLNVDTPFP LEEELQIEPL SLDGLNMLSD SSMGLLDPSV EETFRADRL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRTC3重组蛋白的3篇参考文献信息，包含文献名称、作者及摘要内容概括：
1. **文献名称**：*CRTC3 links catecholamine signalling to energy balance*
**作者**：Song Y, et al.
**摘要**：该研究揭示了CRTC3重组蛋白在儿茶酚胺信号通路中的作用，发现其通过调控脂肪细胞中的cAMP/PKA通路影响能量代谢平衡，CRTC3缺失会导致小鼠肥胖和胰岛素抵抗。
2. **文献名称**：*Regulation of hepatic gluconeogenesis by the CREB coactivator CRTC3*
**作者**：Liu Y, et al.
**摘要**：研究利用重组CRTC3蛋白探索其在肝脏糖异生中的功能，发现CRTC3通过磷酸化依赖的核转位激活CREB靶基因(如PGC-1α)，从而调控血糖稳态，并与2型糖尿病相关。
3. **文献名称**：*AMPK-dependent phosphorylation of CRTC3 controls mitochondrial biogenesis and thermogenesis*
**作者**：Chen W, et al.
**摘要**：该研究通过体外重组CRTC3蛋白实验，证明AMPK对CRTC3的磷酸化修饰可抑制其转录活性，进而影响线粒体生成和脂肪组织产热，为肥胖治疗提供潜在靶点。
以上文献均聚焦CRTC3重组蛋白在代谢调控中的分子机制，涉及信号通路、疾病模型及潜在治疗意义。如需具体发表年份或期刊，可进一步补充检索。
CRTC3 (CREB-regulated transcription coactivator 3) is a member of the CRTC family of proteins that act as coactivators for the cAMP response element-binding protein (CREB), a transcription factor central to cellular responses to hormonal and metabolic signals. CRTC proteins, including CRTC1. CRTC2. and CRTC3. regulate gene expression by bridging CREB with other transcriptional machinery, particularly in pathways linked to energy homeostasis, stress adaptation, and circadian rhythms. CRTC3 is distinguished by its tissue-specific expression, with high levels in adipose tissue, liver, and skeletal muscle, suggesting a specialized role in metabolic regulation.
Recombinant CRTC3 protein is engineered for in vitro studies to elucidate its molecular interactions, post-translational modifications, and regulatory mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein retains functional domains critical for CREB binding and nuclear localization. Researchers utilize it to investigate how CRTC3 modulates target genes involved in gluconeogenesis, lipogenesis, and insulin signaling. Notably, CRTC3 has been implicated in obesity and metabolic syndrome, with studies showing its dysregulation under conditions of nutrient excess or endoplasmic reticulum stress.
Structurally, CRTC3 contains conserved N-terminal CREB-binding domains and regulatory phosphorylation sites responsive to kinases like AMPK or SIK. Recombinant variants often include tags (e.g., His or GFP) for purification or tracking. Current research focuses on CRTC3’s role in adipose inflammation, cancer metabolism, and crosstalk with pathways such as mTOR and Wnt. Its potential as a therapeutic target for metabolic disorders or cancer continues to drive interest in recombinant CRTC3 as a tool for mechanistic and drug-discovery studies.
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