| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL5 |
| Uniprot No | Q96PQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-755aa |
| 活性数据 | MNVIYFPLHI FVVYSRAYTS LVLVGCTNLC AVLFARCLDD HLVSLRMSGS RKEFDVKQIL KIRWRWFGHQ ASSPNSTVDS QQGEFWNRGQ TGANGGRKFL DPCSLQLPLA SIGYRRSSQL DFQNSPSWPM ASTSEVPAFE FTAEDCGGAH WLDRPEVDDG TSEEENESDS SSCRTSNSSQ TLSSCHTMEP CTSDEFFQAL NHAEQTFKKM ENYLRHKQLC DVILVAGDRR IPAHRLVLSS VSDYFAAMFT NDVREARQEE IKMEGVEPNS LWSLIQYAYT GRLELKEDNI ECLLSTACLL QLSQVVEACC KFLMKQLHPS NCLGIRSFAD AQGCTDLHKV AHNYTMEHFM EVIRNQEFVL LPASEIAKLL ASDDMNIPNE ETILNALLTW VRHDLEQRRK DLSKLLAYIR LPLLAPQFLA DMENNVLFRD DIECQKLIME AMKYHLLPER RPMLQSPRTK PRKSTVGTLF AVGGMDSTKG ATSIEKYDLR TNMWTPVANM NGRRLQFGVA VLDDKLYVVG GRDGLKTLNT VECYNPKTKT WSVMPPMSTH RHGLGVAVLE GPMYAVGGHD GWSYLNTVER WDPQARQWNF VATMSTPRST VGVAVLSGKL YAVGGRDGSS CLKSVECFDP HTNKWTLCAQ MSKRRGGVGV TTWNGLLYAI GGHDAPASNL TSRLSDCVER YDPKTDMWTA VASMSISRDA VGVCLLGDKL YAVGGYDGQA YLNTVEAYDP QTNEWTQVAP LCLGRAGACV VTVKL |
| 分子量 | 84.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人KLHL5蛋白的虚拟参考文献框架(注：实际文献可能与此不同，建议通过PubMed或Google Scholar核实)：
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1. **文献名称**: "Structural analysis of recombinant human KLHL5-CUL3 ubiquitin ligase complex"
**作者**: Smith J et al. (2020)
**摘要**: 研究利用冷冻电镜解析了重组人KLHL5蛋白与CUL3的结合结构，揭示了KLHL5通过BTB结构域与CUL3相互作用，并通过Kelch重复序列识别底物的分子机制，为泛素化调控通路提供新见解。
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2. **文献名称**: "Recombinant KLHL5 expression and its role in oxidative stress response"
**作者**: Zhang L et al. (2018)
**摘要**: 开发了重组人KLHL5蛋白的高效大肠杆菌表达系统，证实其通过调控Nrf2通路的泛素化降解参与细胞抗氧化应激反应，揭示了KLHL5在氧化损伤相关疾病中的潜在作用。
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3. **文献名称**: "KLHL5 interacts with synaptic proteins via recombinant human protein screening"
**作者**: Tanaka K et al. (2021)
**摘要**: 利用重组KLHL5蛋白进行酵母双杂交筛选，发现其与突触后蛋白PSD-95的相互作用，提示KLHL5可能通过调节突触蛋白稳定性影响神经元功能，为神经退行性疾病研究提供线索。
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**提示**：若需真实文献，建议在学术数据库中以“KLHL5 recombinant protein”“KLHL5 ubiquitin ligase”或“KLHL5 structure/function”为关键词检索，可重点关注《Journal of Biological Chemistry》《Cell Reports》等期刊的近期文章。
Recombinant human KLHL5 protein is a engineered form of the Kelch-like family member 5 (KLHL5) protein, produced using recombinant DNA technology. KLHL5 is a scaffold protein containing a conserved BTB (BROAD-COMPLEX, TRAMTRACK, AND BRIC-A-BRAC) domain at its N-terminus and multiple Kelch repeat motifs at the C-terminus. These structural features enable KLHL5 to interact with other proteins, notably serving as a substrate-specific adaptor for Cullin-RING E3 ubiquitin ligase complexes (CRLs), which mediate targeted protein ubiquitination and degradation.
KLHL5 is involved in cellular processes such as cell cycle regulation, DNA damage response, and neuronal function. Its role in ubiquitination pathways links it to maintaining protein homeostasis and signaling cascades. Recombinant KLHL5 is widely used in biochemical studies to explore protein-protein interactions, enzymatic mechanisms, and substrate identification. Researchers employ it to dissect its biological functions, particularly in cancer and neurodegenerative diseases where ubiquitination pathways are dysregulated.
Produced in systems like *E. coli* or mammalian cells, recombinant KLHL5 retains functional domains for binding partners, such as CUL3 and specific substrates. Its applications include structural studies (e.g., X-ray crystallography), in vitro ubiquitination assays, and drug discovery targeting ubiquitin-proteasome pathways. Understanding KLHL5's molecular interactions may reveal therapeutic strategies for diseases associated with protein misfolding or aberrant degradation.
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