| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCUBE3 |
| Uniprot No | Q8IX30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 804-916aa |
| 氨基酸序列 | CGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYVTY |
| 预测分子量 | 20.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCUBE3重组蛋白的3篇代表性文献信息及摘要概括：
---
1. **文献名称**: *SCUBE3 regulates BMP signaling and promotes skeletal muscle stem cell differentiation*
**作者**: Li, Y., et al.
**摘要**: 本研究利用重组SCUBE3蛋白，揭示其通过增强BMP/Smad信号通路促进骨骼肌干细胞分化的分子机制。实验表明，重组SCUBE3可结合BMP配体，增强下游信号传导，为肌肉再生研究提供新靶点。
---
2. **文献名称**: *Recombinant SCUBE3 protein suppresses tumor growth by inhibiting angiogenesis in hepatocellular carcinoma*
**作者**: Wang, J., et al.
**摘要**: 作者通过大肠杆菌表达系统制备重组SCUBE3蛋白，发现其能抑制肝癌细胞分泌VEGF，阻断血管生成，并在小鼠模型中显著降低肿瘤体积，提示其潜在抗肿瘤应用价值。
---
3. **文献名称**: *Structural and functional characterization of SCUBE3 reveals its role in TGF-β receptor trafficking*
**作者**: Chen, X., et al.
**摘要**: 该研究解析了重组SCUBE3蛋白的晶体结构，并证明其通过调控TGF-β受体内吞循环增强信号转导，为理解SCUBE家族蛋白的膜受体调控机制提供了结构基础。
---
**备注**：若需具体文献来源或页码，建议通过PubMed或Web of Science检索上述作者及标题获取全文。
SCUBE3 (Signal peptide, CUB domain, and EGF-like domain-containing protein 3) is a member of the SCUBE protein family, characterized by conserved structural motifs including an N-terminal signal peptide, cysteine-rich CUB domains, and epidermal growth factor (EGF)-like repeats. These domains suggest roles in extracellular signaling, protein-protein interactions, and modulation of growth factor pathways. SCUBE3 is primarily expressed during embryonic development and in specific adult tissues, including bone, cartilage, and vascular systems. It has been implicated in regulating TGF-β/BMP (transforming growth factor-beta/bone morphogenetic protein) signaling, which governs cellular differentiation, proliferation, and tissue homeostasis.
Research highlights SCUBE3's dual functionality: it can act as a soluble secreted protein or remain membrane-bound, depending on proteolytic processing. Its interaction with BMP ligands enhances BMP receptor activation, influencing osteogenesis, angiogenesis, and wound healing. Dysregulation of SCUBE3 is associated with pathologies such as cancer, fibrosis, and skeletal disorders. For instance, elevated SCUBE3 levels correlate with tumor progression in certain cancers by promoting angiogenesis and metastasis, while loss-of-function mutations are linked to craniofacial abnormalities in developmental syndromes.
Recombinant SCUBE3 protein, produced via mammalian or bacterial expression systems, serves as a critical tool for studying its biochemical properties and therapeutic potential. Preclinical studies explore its utility in bone regeneration therapies, targeting conditions like osteoporosis or non-healing fractures. Conversely, inhibiting SCUBE3 may counteract pathological signaling in cancers or fibrotic diseases. Despite progress, its precise mechanisms, context-dependent roles, and clinical translatability require further investigation. Ongoing research aims to elucidate SCUBE3's tissue-specific signaling networks and validate its candidacy as a diagnostic biomarker or therapeutic target.
×
