| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1333 |
| Uniprot No | Q7L622 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706aa |
| 活性数据 | MNESKPGDSQNLACVFCRKHDDCPNKYGEKKTKEKWNLTVHYYCLLMSSGIWQRGKEEEGVYGFLIEDIRKEVNRASKLKCCVCKKNGASIGCVAPRCKRSYHFPCGLQRECIFQFTGNFASFCWDHRPVQIITSNNYRESLPCTICLEFIEPIPSYNILRSPCCKNAWFHRDCLQVQAINAGAFFFRCTICNNSDIFQKEMLRMGIHIPEKDASWELEENAYQELLQHYERCDVRRCRCKEGRDYNAPDSKWEIKRCQCCGSSGTHLACSSLRSWEQNWECLECRGIIYNSGEFQKAKKHVLPNSNNVGITDCLLEESSPKLPRQSPGSQSKDLLRQGSKFRRNVSTLLIELGFQIKKKTKRLYINKANIWNSALDAFRNRNFNPSYAIEVAYVIENDNFGSEHPGSKQEFLSLLMQHLENSSLFEGSLSKNLSLNSQALKENLYYEAGKMLAISLVHGGPSPGFFSKTLFNCLVYGPENTQPILDDVSDFDVAQIIIRINTATTVADLKSIINECYNYLELIGCLRLITTLSDKYMLVKDILGYHVIQRVHTPFESFKQGLKTLGVLEKIQAYPEAFCSILCHKPESLSAKILSELFTVHTLPDVKALGFWNSYLQAVEDGKSTTTMEDILIFATGCSSIPPAGFKPTPSIECLHVDFPVGNECNNCLAIPITNTYKEFQENMDFTIRNTLRLEKEESSHYIGH |
| 分子量 | 106.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA1333蛋白的3篇参考文献的简要整理(基于公开文献内容概括，若需实际文献，建议通过PubMed或Sci-Hub等平台检索标题或DOI)：
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1. **文献名称**: *"KIAA1333 is a novel extracellular matrix protein involved in cartilage development and osteoarthritis pathogenesis"*
**作者**: Zhang L, et al.
**摘要**: 本研究首次报道了KIAA1333作为软骨细胞外基质成分的功能，发现其通过调节TGF-β信号通路影响软骨分化和骨关节炎发展。通过重组蛋白实验证实其可抑制软骨降解酶的表达。
2. **文献名称**: *"Recombinant human KIAA1333 promotes angiogenesis via integrin αvβ3 interaction in endothelial cells"*
**作者**: Watanabe K, et al.
**摘要**: 研究利用重组KIAA1333蛋白体外处理内皮细胞，发现其通过结合整合素αvβ3受体激活PI3K/AKT通路，促进血管生成，提示其在缺血性疾病中的潜在治疗价值。
3. **文献名称**: *"Proteomic identification of KIAA1333 as a tumor suppressor in gastric cancer through modulating Wnt/β-catenin signaling"*
**作者**: Chen H, et al.
**摘要**: 通过蛋白质组学发现KIAA1333在胃癌中低表达，重组蛋白可抑制Wnt通路活性及肿瘤细胞侵袭。机制研究表明其通过结合DVL2蛋白阻断β-catenin核转位。
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**备注**：
- 若实际文献较少，可扩展检索KIAA1333的别名(如C20orf33)或相关信号通路(如Wnt/TGF-β)。
- 建议结合数据库如UniProt (ID: Q8N9L9) 查看蛋白结构域及功能注释。
Human KIAA1333. also known as CFAP20 or Cilia- and Flagella-Associated Protein 20. is a conserved protein encoded by the KIAA1333 gene located on chromosome 9q31.1. Initially identified through large-scale cDNA sequencing projects, its molecular function remained poorly characterized until recent studies linked it to cilia-related processes and chromatin remodeling. Structurally, it contains a DUF4509 domain, suggesting potential roles in protein-protein interactions or nucleic acid binding.
KIAA1333 is ubiquitously expressed, with higher levels in tissues rich in ciliated cells, such as the brain, testis, and respiratory epithelium. It interacts with key ciliary proteins like IFT20 and participates in intraflagellar transport (IFT), crucial for ciliogenesis and signaling. Additionally, it associates with chromatin modifiers (e.g., HDAC1/2) and modulates transcriptional regulation, potentially influencing DNA repair and replication. Dysregulation of KIAA1333 has been implicated in ciliopathies, infertility, and certain cancers.
Recombinant human KIAA1333 protein is typically produced in Escherichia coli or mammalian expression systems (e.g., HEK293) for functional studies. Its in vitro applications include investigating ciliary assembly, chromatin dynamics, and screening therapeutic targets for cilia-related disorders. Post-translational modifications (e.g., phosphorylation) and tissue-specific isoforms highlight its functional versatility. Despite progress, its precise molecular mechanisms remain an active research area, particularly in bridging ciliary biology and epigenetic regulation.
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