| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1274 |
| Uniprot No | Q9ULE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-856aa |
| 活性数据 | MGTTASTAQQTVSAGTPFEGLQGSGTMDSRHSVSIHSFQSTSLHNSKAKSIIPNKVAPVVITYNCKEEFQIHDELLKAHYTLGRLSDNTPEHYLVQGRYFLVRDVTEKMDVLGTVGSCGAPNFRQVQGGLTVFGMGQPSLLGFRRVLQKLQKDGHRECVIFCVREEPVLFLRADEDFVSYTPRDKQNLHENLQGLGPGVRVESLELAIRKEIHDFAQLSENTYHVYHNTEDLWGEPHAVAIHGEDDLHVTEEVYKRPLFLQPTYRYHRLPLPEQGSPLEAQLDAFVSVLRETPSLLQLRDAHGPPPALVFSCQMGVGRTNLGMVLGTLILLHRSGTTSQPEAAPTQAKPLPMEQFQVIQSFLRMVPQGRRMVEEVDRAITACAELHDLKEVVLENQKKLEGIRPESPAQGSGSRHSVWQRALWSLERYFYLILFNYYLHEQYPLAFALSFSRWLCAHPELYRLPVTLSSAGPVAPRDLIARGSLREDDLVSPDALSTVREMDVANFRRVPRMPIYGTAQPSAKALGSILAYLTDAKRRLRKVVWVSLREEAVLECDGHTYSLRWPGPPVAPDQLETLEAQLKAHLSEPPPGKEGPLTYRFQTCLTMQEVFSQHRRACPGLTYHRIPMPDFCAPREEDFDQLLEALRAALSKDPGTGFVFSCLSGQGRTTTAMVVAVLAFWHIQGFPEVGEEELVSVPDAKFTKGEFQVVMKVVQLLPDGHRVKKEVDAALDTVSETMTPMHYHLREIIICTYRQAKAAKEAQEMRRLQLRSLQYLERYVCLILFNAYLHLEKADSWQRPFSTWMQEVASKAGIYEILNELGFPELESGEDQPFSRLRYRWQEQSCSLEPSAPEDLL |
| 分子量 | 123.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人KIAA1274蛋白的模拟参考文献示例(请注意，这些文献为虚构，仅用于展示格式和内容参考)：
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1. **文献名称**: *Expression and Purification of Recombinant Human KIAA1274 Protein in E. coli*
**作者**: Zhang L, et al.
**摘要**: 本研究描述了在大肠杆菌中高效表达重组人KIAA1274蛋白的优化方法，通过His标签亲和层析纯化蛋白，并验证了其正确折叠及可溶性。该研究为后续功能研究提供了材料基础。
2. **文献名称**: *KIAA1274 Interacts with Microtubule-Associated Proteins in Neuronal Development*
**作者**: Tanaka M, et al.
**摘要**: 通过免疫共沉淀实验发现，重组KIAA1274蛋白与微管相关蛋白(如MAP1B)存在相互作用，提示其在神经元轴突生长和突触形成中可能起调控作用。
3. **文献名称**: *Loss of KIAA1274 Function Associates with Hirschsprung Disease Pathogenesis*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组KIAA1274蛋白进行体外功能实验，发现其在肠神经系统发育中通过调控RET信号通路发挥作用，基因缺失与先天性巨结肠症相关。
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**注意事项**：
- 上述文献及内容均为示例，真实文献需通过PubMed、Google Scholar等平台检索(可用关键词：KIAA1274、GARRE1、recombinant KIAA1274)。
- KIAA1274蛋白的别名可能包括“GARRE1”(根据UniProt数据库)，建议扩展检索词。
KIAA1274. also known as C12ORF4. is a human gene encoding a protein with incompletely characterized functions. Initially identified through large-scale cDNA sequencing projects, the KIAA1274 gene is located on chromosome 12q24.31 and encodes a conserved 583-amino-acid polypeptide. Bioinformatic analyses suggest structural motifs, including coiled-coil domains, which may mediate protein-protein interactions. Limited studies indicate its potential involvement in cellular processes such as cytoskeletal organization, intracellular trafficking, and signal transduction. Research has linked KIAA1274 to neural development, with murine orthologs showing expression patterns in the nervous system. A notable study demonstrated its interaction with Huntingtin-associated protein 1 (HAP1), suggesting a possible role in Huntington's disease pathology. Recombinant human KIAA1274 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, antibody production, and interaction analyses. Current research gaps include detailed mechanistic insights into its cellular roles and pathophysiological relevance. The recombinant protein serves as a critical tool for elucidating its post-translational modifications, interactome mapping, and structural characterization. Understanding KIAA1274 functions could provide new perspectives on neurological disorders and cellular regulatory networks.
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