| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DTX1 |
| Uniprot No | Q86Y01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-620aa |
| 氨基酸序列 | MSRPGHGGLM PVNGLGFPPQ NVARVVVWEW LNEHSRWRPY TATVCHHIEN VLKEDARGSV VLGQVDAQLV PYIIDLQSMH QFRQDTGTMR PVRRNFYDPS SAPGKGIVWE WENDGGAWTA YDMDICITIQ NAYEKQHPWL DLSSLGFCYL IYFNSMSQMN RQTRRRRRLR RRLDLAYPLT VGSIPKSQSW PVGASSGQPC SCQQCLLVNS TRAASNAILA SQRRKAPPAP PLPPPPPPGG PPGALAVRPS ATFTGAALWA APAAGPAEPA PPPGAPPRSP GAPGGARTPG QNNLNRPGPQ RTTSVSARAS IPPGVPALPV KNLNGTGPVH PALAGMTGIL LCAAGLPVCL TRAPKPILHP PPVSKSDVKP VPGVPGVCRK TKKKHLKKSK NPEDVVRRYM QKVKNPPDED CTICMERLVT ASGYEGVLRH KGVRPELVGR LGRCGHMYHL LCLVAMYSNG NKDGSLQCPT CKAIYGEKTG TQPPGKMEFH LIPHSLPGFP DTQTIRIVYD IPTGIQGPEH PNPGKKFTAR GFPRHCYLPN NEKGRKVLRL LITAWERRLI FTIGTSNTTG ESDTVVWNEI HHKTEFGSNL TGHGYPDASY LDNVLAELTA QGVSEAAAKA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DTX1重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **标题**: "Recombinant DTX1 Protein Expression and Functional Analysis in Notch Signaling"
**作者**: Li Y, Chen H, Wang J
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组DTX1蛋白，验证其作为E3泛素连接酶在Notch信号通路中的功能，证明其通过泛素化修饰调控下游靶蛋白的降解。
2. **标题**: "Structural Characterization of Human DTX1 by X-ray Crystallography"
**作者**: Smith RK, Johnson L, Tanaka M
**摘要**: 首次解析了重组人源DTX1蛋白的晶体结构，揭示其N末端结构域与底物结合的关键位点，为靶向DTX1的分子机制研究提供结构基础。
3. **标题**: "DTX1 Recombinant Protein Enhances T Cell Activation in Autoimmunity Models"
**作者**: Gupta S, Patel V, Lee C
**摘要**: 利用哺乳动物细胞表达系统制备功能性重组DTX1蛋白，发现其通过调控T细胞受体信号传导通路，在自身免疫疾病模型中调节T细胞过度活化。
4. **标题**: "Development of a DTX1-Based Inhibitor Screening Platform Using Recombinant Protein"
**作者**: Zhang Q, Liu T, Zhou X
**摘要**: 构建基于重组DTX1蛋白的高通量药物筛选体系，筛选出小分子化合物可阻断DTX1与Notch1胞内结构域的相互作用，为癌症治疗提供潜在策略。
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**注**：以上为模拟文献，如需真实文献，建议通过PubMed或Google Scholar搜索关键词“DTX1 recombinant protein”或“Deltex1 expression”获取。近年来关于DTX1的研究多集中于其与Notch信号、免疫调控及肿瘤发生的关系。
**Background of DTX1 Recombinant Protein**
DTX1 (Deltex1) is a member of the Deltex protein family, initially identified as a cytoplasmic effector of the Notch signaling pathway, a highly conserved pathway critical for cell fate determination, differentiation, and development. The Notch pathway regulates diverse biological processes, including immune cell activation, stem cell maintenance, and tissue homeostasis. DTX1 functions as an E3 ubiquitin ligase, mediating protein ubiquitination and degradation, and plays a dual role in Notch signaling—either enhancing or inhibiting its activity depending on cellular context.
Structurally, DTX1 contains conserved domains, such as an N-terminal Deltex-specific domain involved in protein interactions and a C-terminal RING finger domain essential for its E3 ligase activity. Its recombinant form is typically engineered using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, interaction partners, and regulatory mechanisms. Recombinant DTX1 protein is purified with tags (e.g., His, GST) for experimental applications, including *in vitro* ubiquitination assays, binding studies, and structural analyses.
Research highlights DTX1's involvement in immune regulation, particularly in T-cell and B-cell development, as well as its role in cancers, where dysregulated Notch signaling is common. Overexpression or mutations in DTX1 have been linked to malignancies like T-cell acute lymphoblastic leukemia (T-ALL) and solid tumors. Additionally, DTX1 interacts with non-Notch pathways, such as Wnt and Hippo, suggesting broader regulatory functions.
The availability of recombinant DTX1 protein facilitates drug discovery and mechanistic studies, offering insights into its therapeutic potential as a target for modulating Notch-related diseases. Its study also contributes to understanding post-translational modifications and protein stability in cellular signaling networks.
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