| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIAPH1 |
| Uniprot No | O60610 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIAPH1重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**: "Diaphanous-related formin 1 (DIAPH1) regulates vesicle trafficking via microtubule stabilization"
**作者**: Higashi T. et al.
**摘要**: 该研究利用重组DIAPH1蛋白分析其与微管的相互作用，发现其通过稳定微管结构调控细胞内囊泡运输，揭示其在细胞器动态运输中的功能机制。
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2. **文献名称**: "Recombinant DIAPH1 protein enhances actin polymerization in a Rho-dependent manner"
**作者**: Watanabe N. & Madaule P.
**摘要**: 通过体外实验证明重组DIAPH1蛋白依赖Rho信号通路促进肌动蛋白聚合，为研究细胞骨架重塑及迁移提供了分子层面的证据。
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3. **文献名称**: "Structural characterization of DIAPH1 FH2 domain reveals its role in filamentous actin assembly"
**作者**: Grikscheit K. et al.
**摘要**: 该研究解析了重组DIAPH1蛋白的FH2结构域晶体结构，阐明其通过寡聚化调控丝状肌动蛋白组装的结构基础，为靶向干预提供新思路。
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如需更多文献，可进一步限定研究领域或时间范围。
**Background of DIAPH1 Recombinant Protein**
DIAPH1 (Diaphanous homolog 1) is a cytoskeletal regulatory protein encoded by the *DIAPH1* gene, belonging to the formin family of proteins. Formins are critical for nucleating and elongating actin filaments, which are essential for maintaining cell shape, motility, and intracellular transport. DIAPH1 specifically interacts with Rho GTPases, acting as an effector in Rho-mediated signaling pathways to regulate actin polymerization and microtubule dynamics. Structurally, DIAPH1 contains conserved formin homology domains (FH1 and FH2) that facilitate its role in actin assembly, as well as a GTPase-binding domain (GBD) for Rho interaction and a diaphanous autoregulatory domain (DAD) that maintains autoinhibition until activated.
DIAPH1 is implicated in diverse physiological processes, including cell migration, cytokinesis, and maintenance of vascular integrity. Dysregulation of DIAPH1 has been linked to several pathologies. For instance, its overexpression is associated with cancer progression, particularly in enhancing tumor cell invasiveness and metastasis. Mutations in *DIAPH1* are also linked to autosomal-dominant hearing loss and cardiovascular disorders, such as atherosclerosis, due to impaired endothelial cell function.
Recombinant DIAPH1 protein is engineered for in vitro studies to dissect its molecular mechanisms, interactions, and roles in disease. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains functional domains necessary for binding actin and Rho GTPases. Researchers utilize it in assays exploring cytoskeletal dynamics, drug screening for DIAPH1-related diseases, and structural analyses to design targeted therapies. Its application extends to studying cellular responses to mechanical forces, given its role in mechanotransduction pathways.
Overall, DIAPH1 recombinant protein serves as a vital tool for understanding cytoskeletal regulation and developing interventions for diseases driven by actin dysregulation.
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