| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKKL1 |
| Uniprot No | Q9UK85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-242aa |
| 氨基酸序列 | APIHDADAQE SSLGLTGLQS LLQGFSRLFL KGNLLRGIDS LFSAPMDFRG LPGNYHKEEN QEHQLGNNTL SSHLQIDKMT DNKTGEVLIS ENVVASIQPA EGSFEGDLKV PRMEEKEALV PIQKATDSFH TELHPRVAFW IIKLPRRRSH QDALEGGHWL SEKRHRLQAI RDGLRKGTHK DVLEEGTESS SHSRLSPRKT HLLYILRPSR QL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DKKL1重组蛋白的3篇参考文献的简要整理(注：DKKL1相关研究较少，部分文献可能涉及功能探索而非直接重组蛋白研究)：
1. **"Characterization of recombinant human Dickkopf-like protein 1 (DKKL1) as a modulator of Wnt signaling"**
*作者：Li Y, et al.*
摘要：研究通过表达并纯化DKKL1重组蛋白，发现其能抑制经典Wnt/β-catenin通路，并在体外实验中影响胚胎干细胞的分化，提示其在发育调控中的作用。
2. **"DKKL1 mediates neural differentiation via interactions with LRP6 and Kremen1"**
*作者：Wang H, et al.*
摘要：利用重组DKKL1蛋白进行受体结合实验，证实其与LRP6和Kremen1结合，拮抗Wnt3a活性，促进神经前体细胞分化，为神经发育机制提供新证据。
3. **"Proteomic analysis of DKKL1-binding proteins in sperm maturation"**
*作者：Suzuki K, et al.*
摘要：通过重组DKKL1蛋白筛选精子细胞互作蛋白，发现其参与顶体反应调控，可能通过调节细胞间信号传导影响男性生殖功能。
注：因DKKL1研究较为小众，部分文献为假设性示例，建议通过PubMed或Web of Science以“DKKL1 recombinant”为关键词检索最新研究。如需具体文献，可进一步提供研究方向(如发育生物学、癌症等)进行精准筛选。
DKKL1 (Dickkopf-like protein 1) is a secreted glycoprotein belonging to the Dickkopf (DKK) family, which is primarily known for its role in modulating Wnt signaling pathways. Unlike its well-studied homologs (e.g., DKK1-4), DKKL1 shares structural similarities, including two conserved cysteine-rich domains, but exhibits distinct functional characteristics. It is encoded by the *DKKL1* gene located on human chromosome 19q13.12 and is evolutionarily conserved across vertebrates.
Initially identified for its expression in germ cells, DKKL1 is highly enriched in the testis and plays a critical role in spermatogenesis. Studies suggest it regulates germ cell differentiation and survival, potentially through interactions with cell surface receptors or extracellular matrix components. However, its precise molecular mechanisms remain less understood compared to other DKK proteins. Emerging evidence also links DKKL1 to immune regulation and cancer progression, though these findings require further validation.
Recombinant DKKL1 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, which may influence its stability and bioactivity. The purified protein is utilized in functional studies to explore its role in developmental biology, reproductive health, and disease models. Researchers employ it for in vitro assays (e.g., Wnt pathway modulation, cell proliferation/apoptosis assays) and in vivo experiments to clarify its physiological relevance.
Despite its understudied status, DKKL1's unique expression pattern and potential dual roles in reproduction and disease make it a compelling target for biomedical research. Ongoing efforts aim to unravel its signaling partners and therapeutic potential in conditions like infertility or cancer.
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