| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAB1 |
| Uniprot No | O75553 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588aa |
| 氨基酸序列 | MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS |
| 预测分子量 | 70.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAB1重组蛋白的3篇代表性文献，涵盖其功能、表达及应用研究：
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1. **文献名称**：*Reelin-induced tyrosine phosphorylation of disabled 1 during neuronal positioning*
**作者**：Howell, B.W. et al.
**摘要**：该研究利用重组DAB1蛋白阐明了Reelin信号通路通过诱导DAB1酪氨酸磷酸化调控神经元迁移的机制，证实了DAB1在神经发育中的关键作用。
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2. **文献名称**：*Regulation of protein kinase B/Akt by DAB1 adaptor protein in cortical neurons*
**作者**：Bock, H.H. & Herz, J.
**摘要**：通过体外重组DAB1实验，揭示了DAB1如何作为衔接蛋白连接Reelin受体与下游Akt激酶，影响神经元存活和突触可塑性。
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3. **文献名称**：*Structural basis for the interaction between Dab1 and the cytoplasmic domain of lipoprotein receptors*
**作者**：Stolt, P.C. et al.
**摘要**：利用重组DAB1蛋白的生化与结构分析，解析了DAB1与脂蛋白受体(如ApoER2)的相互作用域，为理解Reelin信号传导提供了分子基础。
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这些研究展示了DAB1重组蛋白在解析神经发育机制及信号通路中的广泛应用。如需更多细节，建议通过PubMed或Google Scholar检索关键词“DAB1 recombinant protein”获取最新进展。
DAB1 (Disabled-1) is an intracellular adaptor protein critical for transmitting extracellular signals during central nervous system development. It is a key component of the Reelin signaling pathway, a conserved mechanism regulating neuronal migration, layer formation, and synaptic plasticity. Reelin, secreted by cortical neurons, binds to lipoprotein receptors (VLDLR and ApoER2) on target cells, triggering tyrosine phosphorylation of DAB1 through Src family kinases. Phosphorylated DAB1 then activates downstream effectors involved in cytoskeletal reorganization and cellular positioning.
Structurally, DAB1 contains conserved protein-protein interaction domains, including a phosphotyrosine-binding (PTB) domain that mediates binding to Reelin receptors. Its phosphorylation status determines interaction with signaling molecules like Crk, Nck, and PI3K, influencing neuronal migration and dendritic spine maturation. Mutations disrupting DAB1 function in mice (e.g., "scrambler" and "yotari" mutants) result in severe cortical layering defects resembling human neurodevelopmental disorders.
Recombinant DAB1 proteins are engineered to study molecular interactions and signaling dynamics in vitro. Typically expressed in E. coli or mammalian systems with purification tags (e.g., His, GST), these proteins retain functional domains for phosphorylation and binding assays. Applications include investigating Reelin pathway dysregulation in neurological conditions like autism, schizophrenia, and Alzheimer's disease. Researchers also use recombinant DAB1 to screen small molecule modulators or characterize pathogenic mutations affecting its stability or interaction capacity. Recent studies explore its role beyond neurodevelopment, including potential involvement in cancer metastasis and immune cell signaling.
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