| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DLG3 |
| Uniprot No | Q92796 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-817aa |
| 氨基酸序列 | MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPGGGNGASAGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKSTPKLNGSGPSWWPECTCTNRDWYEQVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVSYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIYDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DLG3重组蛋白的3篇参考文献(基于公开研究数据整理，非真实文献，仅示例格式)：
---
1. **文献名称**：*Structural and Functional Analysis of Recombinant DLG3 Protein in Synaptic Scaffolding*
**作者**：Chen L, et al.
**摘要**：本研究通过原核表达系统成功纯化重组DLG3蛋白，解析其PDZ结构域的晶体结构，并验证其与神经受体蛋白(如NMDA受体)的相互作用，揭示了DLG3在突触后致密区支架形成中的分子机制。
---
2. **文献名称**：*DLG3 Recombinant Expression in HEK293 Cells and Its Role in X-Linked Intellectual Disability*
**作者**：Smith KA, et al.
**摘要**：利用哺乳动物HEK293细胞系统表达重组DLG3蛋白，通过体外功能实验发现特定突变体导致蛋白稳定性下降，阐明了DLG3基因突变与X染色体连锁智力障碍的病理关联。
---
3. **文献名称**：*Development of a DLG3 Recombinant Protein-Based Assay for High-Throughput Drug Screening*
**作者**：Wang Y, et al.
**摘要**：构建重组DLG3蛋白的高效纯化工艺，并基于其与β-catenin的结合特性建立药物筛选平台，为靶向DLG3相关信号通路的抗癌化合物开发提供技术支持。
---
如需真实文献，建议在PubMed或Web of Science中检索关键词“DLG3 recombinant protein”或“DLG3 expression and purification”。
**Background of DLG3 Recombinant Protein**
DLG3 (Discs Large Homolog 3), also known as SAP102 (Synapse-Associated Protein 102), is a member of the membrane-associated guanylate kinase (MAGUK) family. This protein is characterized by its modular domains, including PDZ, SH3. and guanylate kinase (GK) domains, which facilitate protein-protein interactions and scaffold signaling complexes. DLG3 is predominantly expressed in the central nervous system, particularly at postsynaptic densities, where it plays a critical role in synaptic organization, signal transduction, and neuronal development.
Functionally, DLG3 interacts with glutamate receptors, such as NMDA and AMPA receptors, regulating their trafficking, clustering, and synaptic plasticity. It is essential for maintaining synaptic stability and modulating learning and memory processes. Mutations or dysregulation of DLG3 have been linked to neurodevelopmental disorders, including X-linked intellectual disability and autism spectrum disorders, underscoring its importance in cognitive function.
Recombinant DLG3 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional protein for research applications. This recombinant form retains key domains and interaction capabilities, enabling studies on its structural and mechanistic roles in synaptic signaling, disease models, and drug discovery. Researchers utilize DLG3 recombinant protein to investigate its binding partners, post-translational modifications, and involvement in neurological pathologies. Additionally, it serves as a tool for developing targeted therapies aimed at restoring synaptic function in neurodevelopmental and neurodegenerative conditions.
Overall, DLG3 recombinant protein is a vital resource for unraveling the molecular underpinnings of synaptic biology and advancing therapeutic strategies for brain disorders.
×
