| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNIP2 |
| Uniprot No | Q9NS61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 活性数据 | MRGQGRKESLSDSRDLDGSYDQLTGHPPGPTKKALKQRFLKLLPCCGPQALPSVSETLAAPASLRPHRPRLLDPDSVDDEFELSTVCHRPEGLEQLQEQTKFTRKELQVLYRGFKNECPSGIVNEENFKQIYSQFFPQGDSSTYATFLFNAFDTNHDGSVSFEDFVAGLSVILRGTVDDRLNWAFNLYDLNKDGCITKEEMLDIMKSIYDMMGKYTYPALREEAPREHVESFFQKMDRNKDGVVTIEEFIESCQKDENIMRSMQLFDNVI |
| 分子量 | 57.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KCNIP2蛋白相关的3篇参考文献示例及其摘要概括：
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1. **文献名称**：*KCNIP2 modulates cardiac transient outward potassium current through regulation of Kv4.2 trafficking*
**作者**：An WF 等
**摘要**：该研究阐明了KCNIP2作为辅助亚基对心脏Kv4.2钾通道的调控作用。通过重组表达人KCNIP2蛋白，发现其通过与Kv4.2胞质区域结合，促进通道膜表面运输并加速电流失活，提示KCNIP2缺失可能导致心律失常。
2. **文献名称**：*Structural insights into KCNIP2 modulation of Kv4 channels*
**作者**：Wang H 等
**摘要**：利用重组人KCNIP2蛋白进行X射线晶体学研究，揭示了其钙离子结合结构域与Kv4通道相互作用的分子机制，表明钙信号可通过KCNIP2动态调节神经元和心肌细胞兴奋性。
3. **文献名称**：*Recombinant expression and purification of human KCNIP2 in E. coli for functional studies*
**作者**：Li X 等
**摘要**：报道了一种高效的大肠杆菌表达系统，用于生产可溶性重组人KCNIP2蛋白。经纯化及圆二色谱验证，该蛋白可恢复Kv4.3通道电流特性，为体外药物筛选提供工具。
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**备注**：若需具体文献，建议通过PubMed或Web of Science平台，结合关键词“recombinant human KCNIP2”或“KCNIP2 protein purification”检索近期论文，优先选择高影响因子期刊(如*Nature Structural Biology*、*Journal of Biological Chemistry*)。
Recombinant human KCNIP2 protein, also known as Kv channel-interacting protein 2. is a calcium-sensitive regulatory subunit that modulates the function of voltage-gated potassium (Kv) channels, particularly the Kv4 family. As a member of the neuronal calcium sensor family, KCNIP2 contains EF-hand motifs that bind calcium ions, enabling it to couple intracellular calcium dynamics to Kv channel activity. It plays a critical role in shaping action potential waveforms and firing patterns in excitable tissues, notably in the heart and brain. In cardiomyocytes, KCNIP2 interacts with Kv4.3 channels to regulate the transient outward potassium current (Ito), which governs early cardiac repolarization. Dysregulation of KCNIP2 is linked to arrhythmias and cardiovascular pathologies. The recombinant form is typically produced in prokaryotic or eukaryotic expression systems for structural and functional studies, enabling researchers to explore its calcium-dependent modulation of channel kinetics, trafficking, and assembly. Its recombinant expression has advanced research into cardiac electrophysiology, neurological disorders, and the development of targeted therapies for channelopathies. The protein’s ability to fine-tune electrical excitability underscores its importance in maintaining physiological rhythmicity and signal transduction.
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