| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EP300 |
| Uniprot No | Q09472 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 985-1810aa |
| 氨基酸序列 | QAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEE RSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEA LYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEP WQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCC GRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVS LGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEI IWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQN HPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFE EIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVY HEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQ EWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLE ESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVD PDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD RFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDD ESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRV VQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIK |
| 预测分子量 | 125 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EP300重组蛋白的3篇参考文献及其摘要概括：
1. **"Mutations in the EP300 gene in human cancers"**
*Authors: Gayther SA, et al. (2000)*
摘要：研究发现EP300在多种癌症(如结直肠癌、乳腺癌)中存在体细胞突变，其重组蛋白功能缺失导致染色质修饰异常，从而影响肿瘤抑制通路(如p53)的调控。
2. **"Acetylation of p53 by EP300 promotes transcription of pro-apoptotic genes"**
*Authors: Liu L, et al. (2001)*
摘要：通过体外实验证明，重组EP300蛋白可乙酰化p53.增强其与DNA结合能力，激活下游凋亡相关基因(如BAX)的转录，揭示EP300在DNA损伤应答中的关键作用。
3. **"EP300 interacts with MyoD to regulate skeletal muscle differentiation"**
*Authors: Puri PL, et al. (1997)*
摘要：利用重组EP300蛋白和体外共沉淀实验，发现EP300与转录因子MyoD直接结合，通过乙酰化组蛋白H3促进肌肉特异性基因(如肌球蛋白)表达，调控成肌细胞分化。
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以上文献涵盖了EP300在癌症、凋亡信号及细胞分化中的功能研究，均涉及重组蛋白在机制探索中的应用。
EP300 recombinant protein is a engineered form of the human EP300 (E1A-binding protein p300) protein, a multifunctional transcriptional coactivator with intrinsic histone acetyltransferase (HAT) activity. Encoded by the *EP300* gene, this 300 kDa nuclear protein plays pivotal roles in chromatin remodeling and transcriptional regulation by acetylating histones (H3K18. H4K5) and non-histone targets (e.g., p53. STAT3), facilitating DNA accessibility and signal-dependent gene activation. It interacts with numerous transcription factors (CREB, HIF-1α, NF-κB) and coordinates cellular processes including proliferation, differentiation, apoptosis, and DNA repair.
Recombinant EP300 is typically produced in mammalian or insect expression systems to preserve post-translational modifications and functional domains: the N-terminal CH1 domain (protein interaction), central HAT domain (catalytic activity), and C-terminal transactivation domain. Its production enables *in vitro* studies on epigenetic mechanisms, protein-DNA interactions, and disease pathways. Mutations in *EP300* are linked to Rubinstein-Taybi syndrome, cancers, and neurological disorders, making the recombinant protein valuable for drug screening (e.g., HAT inhibitors) and mechanistic research. Commercial versions often include affinity tags (e.g., GST, His) for purification and are verified via enzymatic assays, western blotting, or mass spectrometry. As a key node in cellular signaling networks, EP300 recombinant protein serves as an essential tool for decoding transcriptional regulation and developing epigenetic therapies.
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