| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | E2F1 |
| Uniprot No | Q01094 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDPDLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于E2F1重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**：*Functional characterization of recombinant E2F1 protein in cell cycle regulation*
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌系统表达并纯化重组人E2F1蛋白，通过体外实验验证其与靶DNA序列(如Cyclin E启动子)的结合能力，并证明其过表达可诱导细胞周期G1/S期转换异常。
2. **文献名称**：*Structural analysis of E2F1-DNA complex using recombinant protein crystallography*
**作者**：Chen L, et al.
**摘要**：通过重组E2F1蛋白的结晶和X射线衍射分析，揭示了E2F1与DNA结合域的关键氨基酸残基及相互作用模式，为设计靶向E2F1的抑制剂提供结构基础。
3. **文献名称**：*E2F1 recombinant protein delivery suppresses tumor growth in vivo*
**作者**：Wang Y, et al.
**摘要**：开发基于纳米载体的重组E2F1蛋白递送系统，证明其能通过诱导肿瘤细胞凋亡和抑制血管生成，显著抑制小鼠移植瘤模型的肿瘤进展。
4. **文献名称**：*Interaction between recombinant E2F1 and retinoblastoma protein (pRB) in vitro*
**作者**：Garcia R, et al.
**摘要**：利用重组E2F1和pRB蛋白进行体外结合实验，发现磷酸化状态调控两者的相互作用，并阐明了E2F1-pRB复合物在细胞周期退出中的动态调控机制。
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注：以上文献为模拟内容，实际引用时需检索真实数据库(如PubMed、Web of Science)。
E2F1 is a member of the E2F transcription factor family, central to regulating cell cycle progression, DNA repair, and apoptosis. As a recombinant protein, it is engineered for experimental or therapeutic use, typically expressed in vitro using bacterial, insect, or mammalian systems to ensure proper folding and post-translational modifications. Structurally, E2F1 contains a DNA-binding domain, a dimerization domain (for interaction with DP partners), and a transactivation domain responsible for target gene activation. Its activity is tightly controlled by the retinoblastoma (RB) tumor suppressor protein, which binds E2F1 in quiescent cells, inhibiting its function. Phosphorylation of RB during G1/S phase transition releases E2F1. enabling transcription of genes essential for DNA replication (e.g., cyclins, DNA polymerases).
Recombinant E2F1 is widely utilized to study cell cycle dysregulation in cancers, as its overexpression can drive uncontrolled proliferation while also inducing apoptosis under stress conditions. Researchers employ it to investigate interactions with RB-family proteins, chromatin-modifying enzymes, or viral oncoproteins. In drug discovery, it serves as a target for small molecules aiming to restore cell cycle control in malignancies. Additionally, engineered mutants (e.g., phosphorylation-deficient variants) help dissect signaling pathways. Despite its pro-oncogenic role, E2F1’s context-dependent tumor-suppressive functions—mediated through apoptosis induction—highlight its therapeutic complexity. Recombinant E2F1 tools thus remain critical for unraveling its dual roles in cancer biology and developing precision oncology strategies.
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