| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFT52 |
| Uniprot No | Q9Y366 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MEKELRSTILFNAYKKEIFTTNNGYKSMQKKLRSNWKIQSLKDEITSEKLNGVKLWITAGPREKFTAAEFEILKKYLDTGGDVFVMLGEGGESRFDTNINFLLEEYGIMVNNDAVVRNVYHKYFHPKEALVSSGVLNREISRAAGKAVPGIIDEESSGNNAQALTFVYPFGATLSVMKPAVAVLSTGSVCFPLNRPILAFYHSKNQGGKLAVLGSCHMFSDQYLDKEENSKIMDVVFQWLTTGDIHLNQIDAEDPEISDYMMLPYTATLSKRNRECLQESDEIPRDFTTLFDLSIFQLDTTSFHSVIEAHEQLNVKHEPLQLIQPQFETPLPTLQPAVFPPSFRELPPPPLELFDLDETFSSEKARLAQITNKCTEEDLEFYVRKCGDILGVTSKLPKDQQDAKHILEHVFFQVVEFKKLNQEHDIDTSETAFQNNF |
| 分子量 | 76.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IFT52蛋白的3篇参考文献示例(文献信息为综合描述，非真实存在)：
1. **文献名称**：*Structural and functional analysis of human IFT52 in ciliary assembly*
**作者**：Li X, Wang Q, Rosenbaum JL
**摘要**：本研究解析了重组人IFT52蛋白的晶体结构，揭示其与IFT88的相互作用位点，并通过敲低实验证明其在纤毛组装中对IFT复合体B稳定性的关键作用。
2. **文献名称**：*IFT52 phosphorylation regulates Hedgehog signaling via ciliary transport*
**作者**：Basten SG, Giles RH
**摘要**：文章发现重组人IFT52蛋白的磷酸化修饰调控其与Kinesin-II的结合能力，进而影响Hedgehog信号通路的纤毛依赖性运输，可能与多囊肾病相关。
3. **文献名称**：*Recombinant human IFT52 interacts with TULP3 in ciliary trafficking*
**作者**：Mukhopadhyay S, Jackson PK
**摘要**：通过体外pull-down实验和质谱分析，证实重组人IFT52与TULP3直接互作，调控GPCR蛋白向纤毛膜的运输，为纤毛病机制研究提供新视角。
4. **文献名称**：*IFT52 deficiency disrupts spermatogenesis by impairing intraflagellar transport*
**作者**：Tang Z, Yan W
**摘要**：利用重组人IFT52蛋白体外回补实验，发现其缺失导致精子鞭毛内运输障碍，引发雄性小鼠不育，提示IFT52在生殖系统中的关键功能。
(注：以上文献为示例性描述，实际研究中建议通过PubMed或Google Scholar检索具体论文。)
**Background of Recombinant Human IFT52 Protein**
IFT52 (Intraflagellar Transport 52) is a critical component of the IFT-B complex, essential for the assembly and maintenance of cilia and flagella. These microtubule-based organelles function as cellular sensors and motility structures, playing pivotal roles in signaling pathways (e.g., Hedgehog) and developmental processes. IFT52 interacts with other IFT-B subunits (e.g., IFT80. IFT88) to mediate anterograde transport of cargoes from the ciliary base to the tip.
Mutations in IFT52 are linked to ciliopathies, such as skeletal dysplasia, renal dysfunction, and retinal degeneration. Recombinant human IFT52 protein, produced via bacterial or mammalian expression systems, enables structural/functional studies and disease mechanism exploration. Its applications include *in vitro* reconstitution of IFT complexes, interaction assays, and screening for therapeutic compounds targeting cilia-related disorders. Research on recombinant IFT52 advances understanding of ciliary biology and potential treatments for ciliopathies.
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