| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFRD2 |
| Uniprot No | Q12894 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506aa |
| 氨基酸序列 | MAWNSPSRRP VWQGGAPRED GGARGVWLPS SGQVSAQRTG RRLVGLEPTP TGSLTPRPPR PVPGMPRARK GNTLRKGGQR RGGGARSSAQ ADSGSSDDEA ASEARSTASE CPSLLSTTAE DSLGGDVVDE QGQQEDLEEK LKEYVDCLTD KSAKTRQGAL ESLRLALASR LLPDFLLERR LTLADALEKC LKKGKGEEQA LAAAVLGLLC VQLGPGPKGE ELFHSLQPLL VSVLSDSTAS PAARLHCASA LGLGCYVAAA DIQDLVSCLA CLESVFSRFY GLGGSSTSPV VPASLHGLLS AALQAWALLL TICPSTQISH ILDRQLPRLP QLLSSESVNL RIAAGETIAL LFELARDLEE EFVYEDMEAL CSVLRTLATD SNKYRAKADR RRQRSTFRAV LHSVEGGECE EEIVRFGFEV LYMDSWARHR IYAAFKEVLG SGMHHHLQNN ELLRDIFGLG PVLLLDATAL KACKVPRFEK HLYNAAAFKA RTKARSRVRD KRADIL |
| 分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IFRD2蛋白的参考文献示例(注：以下内容为模拟生成，实际文献可能存在差异)：
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1. **文献名称**：*Cloning and Prokaryotic Expression of Human IFRD2 Protein for Functional Studies*
**作者**：Zhang L., Wang Y., et al.
**摘要**：该研究报道了人IFRD2基因的克隆，并利用大肠杆菌系统进行重组表达。通过优化表达条件获得了可溶性蛋白，经镍柱纯化后验证了其与已知相互作用蛋白的结合活性，为后续功能研究奠定基础。
2. **文献名称**：*IFRD2 Regulates Myoblast Differentiation via MAPK Signaling Pathway*
**作者**：Li J., Chen H., et al.
**摘要**：研究团队通过哺乳动物细胞系统(HEK293)表达了重组人IFRD2蛋白，并发现其通过调控MAPK通路影响肌母细胞分化。纯化后的蛋白被用于体外细胞实验，证实其在肌肉发育中的潜在作用。
3. **文献名称**：*Structural Characterization of Recombinant Human IFRD2 Using Circular Dichroism*
**作者**：Smith K., Brown T., et al.
**摘要**：本文利用原核系统表达并纯化了重组IFRD2蛋白，通过圆二色光谱分析其二级结构，揭示了该蛋白在不同pH条件下的构象变化，为理解其分子功能提供结构依据。
4. **文献名称**：*IFRD2 as a Novel Immune Modulator: Insights from Recombinant Protein-Based Assays*
**作者**：Garcia R., Lee S., et al.
**摘要**：研究通过昆虫细胞-杆状病毒系统生产重组IFRD2.证明其通过调控干扰素相关通路影响巨噬细胞的免疫应答，提示其在炎症性疾病中的潜在应用价值。
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**备注**：如需具体文献，建议通过PubMed或Web of Science以关键词“recombinant IFRD2”、“human IFRD2 expression”进一步检索。当前针对IFRD2的研究相对较少，可扩展查阅其同源蛋白IFRD1或相关信号通路的研究。
Recombinant human IFRD2 (Interferon-Related Developmental Regulator 2) protein is a synthesized version of the endogenous IFRD2 protein, encoded by the IFRD2 gene located on human chromosome 3. As a member of the interferon-related developmental regulator family, IFRD2 shares structural homology with IFRD1. featuring conserved domains associated with transcriptional regulation and protein-protein interactions. While its exact physiological role remains under investigation, studies suggest IFRD2 is involved in cellular differentiation, proliferation, and apoptosis, particularly in neural and muscular development. It is hypothesized to modulate signaling pathways linked to growth factors, stress responses, and inflammatory cytokines, potentially influencing tissue repair and immune regulation. Dysregulation of IFRD2 has been implicated in certain cancers and neurodegenerative conditions, though mechanistic insights are limited. Recombinant IFRD2 is typically produced using bacterial or mammalian expression systems, enabling studies on its biochemical properties, interaction networks, and therapeutic potential. Purified forms tagged with His or GST facilitate experimental applications, including binding assays and functional analyses. Current research focuses on elucidating its role in cellular adaptation to metabolic or oxidative stress, as well as its interplay with other interferon-related proteins. Its recombinant form serves as a critical tool for deciphering IFRD2's molecular functions and validating its relevance in disease models.
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