| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR5A |
| Uniprot No | P47898 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MDLPVNLTSFSLSTPSPLETNHSLGKDDLRPSSPLLSVFGVLILTLLGFLVAATFAWNLLVLATILRVRTFHRVPHNLVASMAVSDVLVAALVMPLSLVHELSGRRWQLGRRLCQLWIACDVLCCTASIWNVTAIALDRYWSITRHMEYTLRTRKCVSNVMIALTWALSAVISLAPLLFGWGETYSEGSEECQVSREPSYAVFSTVGAFYLPLCVVLFVYWKIYKAAKFRVGSRKTNSVSPISEAVEVKDSAKQPQMVFTVRHATVTFQPEGDTWREQKEQRAALMVGILIGVFVLCWIPFFLTELISPLCSCDIPAIWKSIFLWLGYSNSFFNPLIYTAFNKNYNSAFKNFFSRQH |
| 分子量 | 40.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HTR5A蛋白的3条参考文献示例(注：以下文献信息为虚构，仅供示例参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human HTR5A in HEK293 Cells"*
**作者**: Smith A, et al.
**摘要**: 本研究在HEK293细胞中成功表达重组人HTR5A蛋白，并通过荧光标记和cAMP检测证实其与G蛋白偶联功能。结果显示HTR5A对5-羟色胺的响应具有浓度依赖性，提示其在神经信号传导中的潜在作用。
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2. **文献名称**: *"Cryo-EM Structure of the Human 5-HT5A Receptor in Complex with an Agonist"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜技术解析了重组人HTR5A蛋白与激动剂结合的3.2Å分辨率结构，揭示了其配体结合口袋的独特构象及跨膜螺旋的构象变化，为靶向HTR5A的药物设计提供结构基础。
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3. **文献名称**: *"Development of a High-Yield Purification Protocol for Recombinant HTR5A Using Affinity Chromatography"*
**作者**: Lee JH, et al.
**摘要**: 提出一种优化的重组HTR5A蛋白纯化方法，利用组氨酸标签亲和层析和尺寸排阻色谱，获得高纯度(>95%)的蛋白，适用于体外配体结合实验及高通量药物筛选。
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**备注**：若需真实文献，建议在PubMed或Web of Science中搜索关键词 **"recombinant HTR5A"** 或 **"5-HT5A receptor expression"**，并筛选涉及蛋白表达、纯化、结构或功能研究的文章。
The human serotonin receptor subtype 5A (HTR5A) is a G protein-coupled receptor (GPCR) that binds the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT). It is encoded by the *HTR5A* gene and primarily expressed in the central nervous system, particularly in regions like the hippocampus, cortex, and amygdala. Unlike other serotonin receptors, HTR5A lacks coupling to Gi/o signaling pathways and is proposed to activate Gα proteins, though its precise signaling mechanisms remain under investigation. It is implicated in modulating mood, cognition, and neuropsychiatric conditions, though its functional roles are less characterized compared to other 5-HT receptors.
Recombinant human HTR5A protein is engineered via heterologous expression systems (e.g., HEK293 or insect cells) to enable structural and functional studies. Its production involves cloning the *HTR5A* gene into expression vectors, optimizing conditions for membrane protein stability, and purifying the receptor using affinity chromatography. This recombinant form retains ligand-binding specificity and post-translational modifications critical for receptor activity. Researchers utilize it to screen ligands, map signaling pathways, and explore interactions with potential therapeutics. As dysregulated serotonin signaling is linked to disorders like depression and schizophrenia, HTR5A is a candidate target for neuropharmacology. Structural insights from recombinant protein studies could facilitate the design of selective drugs with fewer side effects. Ongoing research aims to clarify its physiological roles and therapeutic potential in neuropsychiatric diseases.
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