| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPD1 |
| Uniprot No | P10809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-573aa |
| 氨基酸序列 | MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF |
| 分子量 | 88.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4条关于重组人HSPD1蛋白的参考文献及相关摘要的示例：
1. **"Mitochondrial heat shock protein 60 chaperonine activity requires ATP binding and hydrolysis"**
- **作者**: Cheng, M.Y. et al.
- **摘要**: 研究线粒体HSP60(HSPD1)的分子伴侣功能，通过重组蛋白实验证实其依赖ATP的水解活性促进蛋白质折叠，为理解线粒体蛋白质量控制提供依据。
2. **"Heat shock protein 60 (HSP60) acts as a danger signal by activating dendritic cells via TLR2"**
- **作者**: Osterloh, A. et al.
- **摘要**: 发现重组人HSPD1蛋白可通过结合TLR2受体激活树突状细胞，触发促炎细胞因子分泌，阐明其在先天免疫中的“危险信号”作用。
3. **"Recombinant human HSP60 induces pro-inflammatory responses in peripheral blood mononuclear cells"**
- **作者**: Munk, M.E. et al.
- **摘要**: 通过体外实验证实重组HSPD1能够刺激单核细胞分泌IL-6和TNF-α，提示其在自身免疫疾病(如类风湿性关节炎)中的潜在致病机制。
4. **"Efficient expression and purification of recombinant human HSPD1 for cancer immunotherapy studies"**
- **作者**: Wang, Y. et al.
- **摘要**: 描述在大肠杆菌中高效表达并纯化重组人HSPD1的方法，验证其在增强肿瘤抗原特异性T细胞反应中的应用潜力，为癌症疫苗开发提供技术基础。
**注**：以上文献名称、作者及内容为示例性概括，实际引用需以具体出版物为准。建议通过数据库(如PubMed、Google Scholar)以关键词“recombinant human HSPD1”、“HSP60 expression”等检索最新或经典研究。
Recombinant human HSPD1 (Heat Shock Protein Family D1), also known as HSP60 or chaperonin 60. is a mitochondrial protein belonging to the heat shock protein family. It plays a critical role in maintaining cellular proteostasis by assisting in the folding and assembly of newly synthesized mitochondrial polypeptides under both normal and stress conditions. Structurally, HSPD1 forms a large oligomeric complex that collaborates with co-chaperonin HSPE1 (HSP10) to encapsulate substrate proteins in an ATP-dependent manner, facilitating proper conformation. Beyond its molecular chaperone function, HSPD1 is implicated in immune regulation, apoptosis, and cellular signaling pathways. Dysregulation of HSPD1 has been linked to various diseases, including cancer, neurodegenerative disorders (e.g., Parkinson’s disease), and autoimmune conditions like rheumatoid arthritis. Recombinant HSPD1 is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modifications. This engineered protein serves as a vital tool for studying mitochondrial protein quality control mechanisms, disease pathogenesis, and therapeutic development. Additionally, it has potential applications as an antigen for diagnostic assays, a target for drug screening, and a candidate for immunomodulatory therapies. Research continues to explore its dual role in cell survival and stress-induced apoptosis, highlighting its therapeutic promise in precision medicine.
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