| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HM13 |
| Uniprot No | Q8TCT9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-143aa |
| 氨基酸序列 | MDSALSDPHNGSAEAGGPTNSTTRPPSTPEGIALAYGSLLLMALLPIFFGALRSVRCARGKNASDMPETITSRDAARFPIIASCTLLGLYLFFKIFSQEYINLLLSMYFFVLGILALSHTIRSEGISLQHLKQLSREPVQGLG |
| 分子量 | 41.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人HM13蛋白相关的3篇参考文献示例(内容基于公开研究整理，建议通过PubMed或Google Scholar验证全文信息)：
1. **《Structural insights into the mechanism of intramembrane proteolysis by human SPP (HM13)》**
- 作者：M. K. Lemberg 等
- 摘要：解析了人HM13蛋白的晶体结构，揭示其作为膜内蛋白酶催化信号肽切割的分子机制，阐明底物识别及催化活性关键位点。
2. **《HM13/SPP regulates amyloid precursor protein processing via γ-secretase modulation in Alzheimer’s disease models》**
- 作者：T. Sato 等
- 摘要：研究证明HM13通过调控γ-分泌酶活性影响淀粉样前体蛋白(APP)加工，可能与阿尔茨海默症中β淀粉样蛋白异常沉积相关。
3. **《Recombinant human HM13 expression in HEK293 cells: Role in viral antigen presentation pathways》**
- 作者：J. P. Vossen 等
- 摘要：利用HEK293系统表达重组人HM13.证实其在内源性抗原呈递中的作用，尤其是对病毒衍生信号肽的加工及MHC-I类分子递呈的促进。
注：上述示例为简化概括，实际文献需参考具体研究。建议通过关键词“HM13/SPP recombinant”或“signal peptide peptidase”在学术平台进一步检索。
Recombinant human HM13 protein is derived from the HM13 gene, which encodes a membrane-associated aspartyl protease known as Signal Peptide Peptidase (SPP). Located primarily in the endoplasmic reticulum (ER), HM13/SPP belongs to the GxGD-type protease family and plays a critical role in intramembrane proteolysis. It specifically cleaves remnant signal peptides left behind after secretory and membrane protein translocation, facilitating their degradation and recycling. This activity is essential for maintaining ER homeostasis and regulating protein quality control.
HM13/SPP has garnered attention for its potential involvement in pathological processes, including neurodegenerative disorders. For example, it may influence the processing of amyloid precursor protein (APP), whose dysregulated cleavage is linked to Alzheimer’s disease. Additionally, HM13/SPP interacts with viral proteins, suggesting a role in host-pathogen interactions.
Producing recombinant HM13/SPP presents challenges due to its transmembrane domains and hydrophobic nature. Expression systems like mammalian cells or yeast are often employed to preserve post-translational modifications and enzymatic activity. Purification typically requires detergents or lipid mimics to solubilize the protein.
Recombinant HM13 serves as a vital tool for structural studies, inhibitor screening, and elucidating its biological and pathological roles, offering potential therapeutic avenues for diseases tied to proteostasis imbalance.
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