| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DPB2 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MMILQVSGGPWTVALTALLMVLLISVVQSRATPENSVYQERQECYAFNGTQRVVDGLIYNREEYVHFDSAVGEFLAVMELGRPIGEYFNSQKDFMERKRAEVDKVCRHKYELMEPLIRQRRGDVTITAVRGCWTTILSGYFLLKRGVVSGGCSWGSS |
| 分子量 | 44.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HLA-DPB2蛋白的参考文献示例(注：以下内容为假设性生成，实际文献需通过学术数据库验证)：
1. **"Expression and Purification of Recombinant HLA-DPB2 in Mammalian Cells"**
- **作者**: Smith A. et al.
- **摘要**: 该研究描述了在HEK293细胞中成功表达重组人HLA-DPB2蛋白的方法，通过亲和层析纯化获得高纯度蛋白，并证实其与β2微球蛋白形成稳定复合物，可用于后续免疫学研究。
2. **"Structural Analysis of HLA-DPB2 Reveals Distinct Antigen-Binding Features"**
- **作者**: Tanaka K. et al.
- **摘要**: 通过X射线晶体学解析HLA-DPB2的三维结构，发现其抗原结合沟的氨基酸残基组成与其他HLA-II类分子(如HLA-DR和HLA-DQ)存在差异，可能影响抗原呈递的特异性。
3. **"HLA-DPB2 Genetic Variants and Autoimmune Disease Susceptibility"**
- **作者**: Chen L. et al.
- **摘要**: 研究分析了HLA-DPB2基因多态性与类风湿性关节炎的关联，发现特定单体型可能通过调节T细胞反应参与自身免疫病理过程。
4. **"Functional Role of HLA-DPB2 in Immune Response Regulation"**
- **作者**: Müller R. et al.
- **摘要**: 利用重组HLA-DPB2蛋白进行体外实验，证明其在树突状细胞介导的T细胞激活中发挥调控作用，可能作为潜在免疫治疗靶点。
**建议**：通过PubMed或Google Scholar检索关键词“HLA-DPB2 recombinant protein”“HLA-DPB2 structure/function”以获取真实文献。
Recombinant human HLA-DPB2 protein is a genetically engineered form of the HLA-DPB2 antigen, a critical component of the human major histocompatibility complex (MHC) class II system. HLA-DPB2. located on chromosome 6. encodes the beta chain of the HLA-DP heterodimer, which plays a central role in antigen presentation to CD4+ T cells, triggering adaptive immune responses. Unlike HLA-DPB1. which is highly polymorphic and directly involved in peptide binding, HLA-DPB2 is considered a "pseudogene" in some individuals due to regulatory mutations that prevent its expression. However, recent studies suggest it may retain functional significance in certain haplotypes or contexts.
Recombinant HLA-DPB2 is typically produced using expression systems like mammalian cells or bacteria, enabling controlled study of its structural and immunological properties. Its production facilitates research into HLA-DP complex assembly, antigen processing mechanisms, and the functional impact of HLA-DPB2 variants in diseases such as autoimmune disorders, graft-versus-host disease, and infectious immunity. Additionally, recombinant HLA-DPB2 serves as a tool for investigating allelic exclusion patterns between DPB1 and DPB2. as well as potential roles in T-cell repertoire shaping. Its availability supports the development of diagnostic reagents and therapeutic strategies targeting MHC class II pathways. Current research continues to explore whether DPB2's limited polymorphism and variable expression across populations influence immune response diversity.
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