| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DQA1 |
| Uniprot No | P01909 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-213aa |
| 氨基酸序列 | EDIVADHVASYGVNLYQSYGPSGQYTHEFDGDEQFYVDLGRKETVWCLPVLRQFRFDPQFALTNIAVLKHNLNSLIKRSNSTAATNEVPEVTVFSKSPVTLGQPNILICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSE |
| 分子量 | 25.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HLA-DQA1蛋白的3篇参考文献的简要信息：
1. **文献名称**: Expression and Purification of Functional Recombinant HLA-DQA1*05:01 Chain
**作者**: Smith J, et al.
**摘要**: 报道了一种通过哺乳动物细胞表达系统高效表达重组HLA-DQA1*05:01蛋白的方法，验证了其与HLA-DQB1形成的异源二聚体在抗原呈递中的功能性，为研究乳糜泻等疾病的分子机制提供了工具。
2. **文献名称**: Structural Insights into HLA-DQA1 Polymorphism and Peptide Binding Specificity
**作者**: Brown K, et al.
**摘要**: 利用X射线晶体学解析了重组HLA-DQA1蛋白的三种常见变异体的三维结构，揭示了不同等位基因对结合肽段选择性的影响，并阐明了与1型糖尿病易感性的潜在关联。
3. **文献名称**: Recombinant HLA-DQA1 as a Tool for Autoantibody Detection in Autoimmune Thyroiditis
**作者**: Zhang L, et al.
**摘要**: 构建了重组HLA-DQA1*03蛋白并用于检测患者血清中的特异性自身抗体，证明其在桥本甲状腺炎诊断中的敏感性和临床价值，为自身免疫疾病的生物标志物研究提供新方法。
注：以上文献信息为模拟示例，实际引用时需以真实文献为准。
Human leukocyte antigen (HLA)-DQA1 is a key component of the major histocompatibility complex (MHC) class II system, responsible for presenting exogenous antigens to CD4+ T-cells to initiate adaptive immune responses. Encoded by the HLA-DQA1 gene on chromosome 6p21.3. it exhibits high polymorphism, with numerous alleles linked to autoimmune diseases, including type 1 diabetes, celiac disease, and rheumatoid arthritis. The HLA-DQA1 protein forms a heterodimer with HLA-DQB1. creating a functional peptide-binding groove critical for antigen presentation. Variations in DQA1 alleles influence peptide-binding specificity, affecting disease susceptibility and immune compatibility in organ transplantation.
Recombinant HLA-DQA1 protein is produced using expression systems (e.g., mammalian, insect, or bacterial cells) to study its structural and immunological roles. It enables investigations into allele-specific antigen interactions, T-cell receptor engagement, and autoimmune mechanisms. Researchers also use it to develop HLA-typing assays, epitope-mapping tools, and therapeutic strategies targeting MHC-mediated pathologies. Its recombinant form bypasses the challenges of isolating natural HLA proteins from human cells, ensuring standardized purity and scalability. Current studies focus on clarifying its role in disease pathways and designing HLA-directed therapies, including tolerogenic vaccines and precision immunomodulators.
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