| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABI1 |
| Uniprot No | Q8IZP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-508aa |
| 氨基酸序列 | AELQMLLEE EIPSGKRALI ESYQNLTRVA DYCENNYIQA TDKRKALEET KAYTTQSLAS VAYQINALAN NVLQLLDIQA SQLRRMESSI NHISQTVDIH KEKVARREIG ILTTNKNTSR THKIIAPANM ERPVRYIRKP IDYTVLDDVG HGVKWLKAKH GNNQPARTGT LSRTNPPTQK PPSPPMSGRG TLGRNTPYKT LEPVKPPTVP NDYMTSPARL GSQHSPGRTA SLNQRPRTHS GSSGGSGSRE NSGSSSIGIP IAVPTPSPPT IGPENISVPP PSGAPPAPPL APLLPVSTVI AAPGSAPGSQ YGTMTRQISR HNSTTSSTSS GGYRRTPSVT AQFSAQPHVN GGPLYSQNSI SIAPPPPPMP QLTPQIPLTG FVARVQENIA DSPTPPPPPP PDDIPMFDDS PPPPPPPPVD YEDEEAAVVQ YNDPYADGDP AWAPKNYIEK VVAIYDYTKD KDDELSFMEG AIIYVIKKND DGWYEGVCNR VTGLFPGNYV ESIMHYTD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABI1重组蛋白的3篇代表性文献及其摘要概括：
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1. **标题**: *"Structure of the WAVE regulatory complex and insights into immune cell function"*
**作者**: Chen Z, et al.
**摘要**: 本研究解析了包含ABI1的重组WAVE复合体的晶体结构，揭示了ABI1通过其N端结构域稳定复合体，并调控Arp2/3介导的肌动蛋白聚合的分子机制。
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2. **标题**: *"ABI1 regulates SOS1 activity and Ras/MAPK signaling via direct interaction"*
**作者**: Smith J, et al.
**摘要**: 文章通过体外实验证明，重组ABI1蛋白可直接结合SOS1(Ras激活因子)，抑制其GEF活性，从而负调控Ras/MAPK信号通路，影响细胞增殖与迁移。
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3. **标题**: *"Phosphorylation of ABI1 by Tyrosine Kinases Modulates Its Role in Actin Remodeling"*
**作者**: Wang L, et al.
**摘要**: 研究利用重组ABI1蛋白进行激酶实验，发现Abelson酪氨酸激酶(c-Abl)对ABI1的磷酸化修饰可增强其与WAVE复合体的结合能力，促进细胞骨架动态重组。
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**备注**：以上文献为示例性质，具体标题和作者可能需要根据实际数据库检索调整。建议通过PubMed或Google Scholar以关键词“ABI1 recombinant protein”或“ABI1 in vitro study”进一步筛选近期研究。
ABI1 (Abelson Interactor 1) is a multifunctional adaptor protein involved in regulating cytoskeletal dynamics, cell signaling, and membrane trafficking. Initially identified as a binding partner of the Abelson tyrosine kinase (c-Abl), ABI1 plays a critical role in the WAVE (Wiskott-Aldrich syndrome protein-family verprolin-homologous protein) regulatory complex, which governs actin polymerization and cell motility. Structurally, ABI1 contains modular domains, including an SH3 domain that mediates protein-protein interactions and a coiled-coil region essential for complex assembly. It acts as a scaffold, integrating signals from pathways like Rac GTPase to coordinate actin remodeling during processes such as cell migration, endocytosis, and immune synapse formation.
Recombinant ABI1 protein is engineered for in vitro studies to dissect its molecular functions, interactions, and structural properties. Produced using expression systems like *E. coli* or mammalian cells, it retains functional domains while enabling high purity and scalability. Researchers utilize recombinant ABI1 to investigate its role in diseases: aberrant ABI1 expression or mutations are linked to cancer metastasis (e.g., breast, lung) due to disrupted cell adhesion/migration, and neurodegenerative disorders like Alzheimer’s, where actin dysregulation affects synaptic plasticity. It also serves as a tool for screening therapeutic agents targeting ABI1-associated pathways. By studying recombinant ABI1. scientists aim to unravel its precise mechanisms in health and disease, offering potential insights for therapeutic strategies.
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