| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AFF1 |
| Uniprot No | P51825 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1210aa |
| 氨基酸序列 | MAAQSSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIASTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AFF1重组蛋白的3篇参考文献示例(注：部分信息基于领域内典型研究整合，若需实际文献请通过学术数据库验证)：
1. **文献名称**：*Structural basis for the interaction between the MLL fusion protein AF10 and AFF1*
**作者**：Li Y. et al.
**摘要**：该研究通过X射线晶体学解析了AFF1与MLL融合伴侣AF10复合物的三维结构，揭示了AFF1在白血病相关融合蛋白中的关键结合域及其调控转录延伸的分子机制。
2. **文献名称**：*AFF1 recruits P-TEFb through its intrinsically disordered regions to promote transcriptional elongation*
**作者**：Chen F.X. et al.
**摘要**：文章发现AFF1通过其内在无序区域招募正性转录延伸因子b(P-TEFb)，形成超级延伸复合物(SEC)，并利用重组蛋白实验证明AFF1的特定结构域在激活RNA聚合酶II延伸中的必要性。
3. **文献名称**：*Mechanistic insights into MLL-AFF1 oncogenic transcriptional complex assembly*
**作者**：Park S.H. et al.
**摘要**：该研究通过体外重组MLL-AFF1融合蛋白，结合生化实验和结构模拟，阐明其异常招募组蛋白甲基转移酶和转录延伸因子导致白血病发生的分子基础。
*注：以上文献信息为模拟示例，具体文献需通过PubMed/Google Scholar以"AFF1 recombinant"、"AFF1 structure"等关键词检索确认。*
**Background of AFF1 Recombinant Protein**
AFF1 (AF4/FMR2 family member 1), also known as AF4. is a key regulatory protein involved in transcriptional elongation and chromatin modification. It belongs to the AFF (AF4/FMR2) family, which shares conserved domains, including serine-rich regions, nuclear localization signals, and transactivation domains. AFF1 gained prominence due to its role in acute leukemias, particularly those associated with chromosomal translocations involving the *MLL* (Mixed Lineage Leukemia) gene. The *MLL-AFF1* fusion, resulting from t(4;11)(q21;q23), is a common driver of aggressive infant acute lymphoblastic leukemia (ALL) and mixed-lineage leukemia, underscoring its pathological significance.
Biologically, AFF1 functions as part of the super elongation complex (SEC), a multi-protein assembly that regulates RNA polymerase II (Pol II) pause-release during transcription. This complex facilitates the transition of paused Pol II into productive elongation, thereby controlling the expression of genes critical for development, cell cycle progression, and oncogenesis. AFF1 interacts with other SEC components, such as ENL, ELL, and P-TEFb, to mediate chromatin remodeling and transcriptional activation.
Recombinant AFF1 protein is engineered using expression systems like *E. coli* or mammalian cells, often tagged for purification and detection. It serves as a vital tool for studying molecular mechanisms of transcriptional regulation, protein-protein interactions, and leukemogenesis. Researchers utilize recombinant AFF1 to dissect its structural domains, map binding partners, and screen inhibitors targeting MLL-AFF1-driven malignancies. Its applications extend to *in vitro* assays, structural studies, and drug development, offering insights into therapeutic strategies for cancers linked to transcriptional dysregulation.
Understanding AFF1's role in both normal and pathological contexts highlights its dual importance in basic biology and clinical research.
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