| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2AFY2 |
| Uniprot No | Q9P0M6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MSGRSGKKKMSKLSRSARAGVIFPVGRLMRYLKKGTFKYRISVGAPVYMAAVIEYLAAEILELAGNAARDNKKARIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGTKGKSETILSPPPEKRGRKATSGKKGGKKSKAAKPRTSKKSKPKDSDKEGTSNSTSEDGPGDGFTILSSKSLVLGQKLSLTQSDISHIGSMRVEGIVHPTTAEIDLKEDIGKALEKAGGKEFLETVKELRKSQGPLEVAEAAVSQSSGLAAKFVIHCHIPQWGSDKCEEQLEETIKNCLSAAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISAHFDDSSASSLKNVYFLLFDSESIGIYVQEMAKLDAK |
| 分子量 | 66.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人H2AFY2蛋白的3条模拟参考文献示例(注：文献为虚构，实际文献需通过PubMed等数据库查询)：
1. **文献名称**：*Expression and Functional Analysis of Recombinant Human H2AFY2 in Chromatin Remodeling*
**作者**：Li et al.
**摘要**：研究报道了H2AFY2重组蛋白在大肠杆菌中的高效表达与纯化，并证实其通过调控组蛋白修饰复合体(如Polycomb家族)参与肿瘤细胞染色质结构的动态重塑。
2. **文献名称**：*H2AFY2 Binds to Satellite Repeats and Promotes Heterochromatin Formation*
**作者**：Garcia-Rivera et al.
**摘要**：通过体外实验验证重组人H2AFY2蛋白优先结合基因组卫星重复序列(如人类1号染色体着丝粒区域)，揭示其在异染色质形成和基因组稳定性维持中的功能。
3. **文献名称**：*MacroH2A2 (H2AFY2) Regulates Cellular Senescence via p53-Dependent Pathways*
**作者**：Wang & Zhang
**摘要**：研究利用重组H2AFY2蛋白进行蛋白质相互作用组分析，发现其通过结合p53并调控下游衰老相关基因(如p21)，影响细胞衰老进程。
**提示**：实际研究中，H2AFY2常被称为macroH2A2.建议结合关键词"macroH2A2"或"histone variant H2AFY2"查询最新文献(如Nature、Cell子刊等)。其功能多涉及胚胎发育、癌症表观遗传调控及衰老等领域。
Recombinant human H2AFY2 protein is a genetically engineered form of the histone variant macroH2A2. belonging to the H2A histone family. MacroH2A2. encoded by the *H2AFY2* gene, is distinguished by its large C-terminal macrodomain, a unique structural feature absent in canonical histones. This domain facilitates interactions with metabolites like poly-ADP-ribose and O-acetyl-ADP-ribose, linking chromatin remodeling to cellular metabolic states. As a component of nucleosomes, macroH2A2 plays roles in epigenetic regulation, including X-chromosome inactivation, heterochromatin stabilization, and transcriptional repression. It is implicated in cellular differentiation, senescence, and tumor suppression, with altered expression observed in cancers and age-related diseases. Recombinant H2AFY2 protein is commonly expressed in *E. coli* or mammalian systems, often fused with affinity tags (e.g., His, GST) for purification. Its production enables biochemical studies, such as binding assays, structural analyses, and screening for small-molecule modulators. Researchers utilize this recombinant protein to investigate macroH2A2’s role in chromatin dynamics, DNA repair, and its potential as a therapeutic target. Its study also advances understanding of how histone variants influence nuclear organization and disease mechanisms.
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