| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H1FX |
| Uniprot No | Q92522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MSVELEEALPVTTAEGMAKKVTKAGGSAALSPSKKRKNSKKKNQPGKYSQLVVETIRRLGERNGSSLAKIYTEAKKVPWFDQQNGRTYLKYSIKALVQNDTLLQVKGTGANGSFKLNRKKLEGGGERRGAPAAATAPAPTAHKAKKAAPGAAGSRRADKKPARGQKPEQRSHKKGAGAKKDKGGKAKKTAAAGGKKVKKAAKPSVPKVPKGRK |
| 分子量 | 48.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人H1FX蛋白的参考文献示例(注：文献内容及作者为虚拟示例，仅供参考)：
1. **“重组人H1FX组蛋白的制备及其核小体结合特性”**(Li et al., 2020)
- 作者通过原核系统表达并纯化了重组人H1FX蛋白，研究其与核小体的相互作用，发现H1FX在染色质高级结构形成中具有独特调控作用。
2. **“H1FX在胚胎干细胞分化中的表观遗传功能分析”**(Wang et al., 2018)
- 利用重组H1FX蛋白进行体外实验，揭示其在胚胎干细胞多能性维持中通过调节特定基因的染色质开放状态发挥作用。
3. **“CRISPR/Cas9介导的H1FX敲除细胞系构建及重组蛋白功能回补研究”**(Zhang et al., 2021)
- 通过重组H1FX蛋白回补实验，验证了H1FX对DNA损伤修复通路的影响，并发现其与其他组蛋白变体的协同效应。
4. **“H1FX的翻译后修饰及其对染色质动态的调控”**(Garcia-Robles et al., 2017)
- 研究重组H1FX蛋白的磷酸化修饰位点，阐明其在细胞周期进程中调控染色体凝聚的功能机制。
(注：以上文献名称和内容为示例，实际需根据具体研究通过数据库检索补充。)
**Background of Recombinant Human H1FX Protein**
The H1FX protein is a member of the histone H1 family, which comprises linker histones critical for stabilizing higher-order chromatin structure. Unlike core histones (H2A, H2B, H3. H4), H1 histones bind to nucleosomal linker DNA, facilitating chromatin compaction and regulating gene expression by modulating chromatin accessibility. H1FX, a subtype of the H1 family, exhibits tissue-specific expression patterns, with notable roles in germ cells, stem cells, and early embryonic development. Studies suggest its involvement in maintaining genomic stability, transcriptional regulation, and epigenetic inheritance.
Recombinant human H1FX protein is produced using biotechnology platforms, such as *E. coli* or mammalian expression systems, to ensure proper post-translational modifications and functionality. Its recombinant form enables precise exploration of H1FX's biochemical properties, DNA-binding dynamics, and interactions with chromatin-modifying enzymes. Researchers utilize this tool to dissect its contributions to cellular differentiation, DNA repair mechanisms, and epigenetic reprogramming.
Due to its link to developmental processes and potential dysregulation in diseases like cancer, recombinant H1FX is also leveraged in drug discovery and mechanistic studies. By providing a purified, standardized protein source, it aids in elucidating H1FX's structural and functional nuances, bridging gaps in understanding chromatin biology and its therapeutic implications.
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