| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2AFB2 |
| Uniprot No | P0C5Z0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MPRRRRRRGSSGAGGRGRTCSRTVRAELSFSVSQVERSLREGHYAQRLSRTAPVYLAAVIEYLTAKVLELAGNEAQNSGERNITPLLLDMVVHNDRLLSTLFNTTTISQVAPGED |
| 分子量 | 12.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的3篇与重组人H2AFB2蛋白功能及制备相关的参考文献摘要(注：由于H2AFB2属于较新的组蛋白变种研究领域，以下文献标题与内容为模拟编写，实际研究需通过专业数据库验证)：
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1. **文献名称**: *"H2A.B.3 (H2AFB2) facilitates chromatin accessibility during spermatogenesis"*
**作者**: Saskia B. et al.
**摘要**: 该研究通过重组人H2AFB2蛋白体外组装核小体，发现其促进染色质解聚的能力，揭示了其在精子发生过程中通过取代常规组蛋白H2A调控基因表达的分子机制。
2. **文献名称**: *"Recombinant production and crystallographic analysis of the human histone variant H2AFB2"*
**作者**: Watanabe T. et al.
**摘要**: 报道了利用昆虫表达系统高效表达重组H2AFB2蛋白，并通过X射线衍射解析其与组蛋白H3/H4复合物的三维结构，为理解其与非典型核小体组装的关系提供结构基础。
3. **文献名称**: *"H2AFB2 promotes DNA repair by enhancing chromatin flexibility in cancer cells"*
**作者**: Li Y. et al.
**摘要**: 利用纯化的重组H2AFB2蛋白进行体外DNA损伤实验，发现其通过降低核小体稳定性提升同源重组修复效率，提示其在肿瘤放射治疗敏感性中的潜在应用价值。
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**说明**：若需真实文献，建议通过PubMed、Web of Science以关键词 "H2AFB2"、"H2A.B.3"、"recombinant histone variant" 检索最新研究。H2AFB2的现有研究多聚焦于其在生殖、癌症及染色质动态中的作用。
Histone H2A-Barr body-deficient 2 (H2AFB2), a member of the H2A histone family, is a testis-specific histone variant implicated in chromatin organization and transcriptional regulation. Unlike canonical H2A histones, H2AFB2 is primarily expressed in post-meiotic male germ cells and is associated with sperm chromatin compaction during spermiogenesis. Its unique sequence, particularly in the C-terminal tail, influences nucleosome stability and DNA packaging, facilitating the replacement of somatic histones with protamines—a critical step for sperm head condensation and genome integrity.
Recombinant human H2AFB2 protein is synthesized via heterologous expression systems (e.g., E. coli or mammalian cells) to study its structural and functional roles. Its production enables in vitro investigations into chromatin remodeling, epigenetic regulation, and sperm development. Research suggests H2AFB2 may interact with transition proteins and chaperones to mediate histone-to-protamine exchange, with dysregulation linked to male infertility. Additionally, aberrant expression in cancers highlights its potential role in genomic instability.
As a tool, recombinant H2AFB2 aids in elucidating mechanisms of germ cell epigenetics and developing diagnostic or therapeutic strategies for reproductive disorders. Studies also leverage it to explore histone variant-specific post-translational modifications and their impact on gene expression. (Word count: 201)
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