| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFAND6 |
| Uniprot No | Q6FIF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MAQETNHSQV PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QNSSNGRISP PATSVSSLSE SLPVQCTDGS VPEAQSALDS TSSSMQPSPV SNQSLLSESV ASSQLDSTSV DKAVPETEDV QASVSDTAQQ PSEEQSKSLE KPKQKKNRCF MCRKKVGLTG FECRCGNVYC GVHRYSDVHN CSYNYKADAA EKIRKENPVV VGEKIQKI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZFAND6重组蛋白的3篇参考文献(示例为模拟内容，实际文献需根据数据库查询)：
1. **文献名称**：*"Recombinant ZFAND6 protein modulates inflammatory signaling in macrophages"*
**作者**：Li, X., et al.
**摘要**：研究利用大肠杆菌表达系统成功纯化重组ZFAND6蛋白，发现其通过抑制NF-κB通路减轻巨噬细胞炎症反应，提示其在免疫调控中的潜在作用。
2. **文献名称**：*"Structural and functional characterization of ZFAND6 as a ubiquitin-binding protein"*
**作者**：Garcia-Ruiz, S., et al.
**摘要**：通过X射线晶体学解析ZFAND6重组蛋白结构，发现其AN1型锌指结构域特异结合泛素链，参与蛋白质降解调控，为癌症治疗提供新靶点。
3. **文献名称**：*"ZFAND6 recombinant protein alleviates oxidative stress in neuronal cells"*
**作者**：Park, J.H., et al.
**摘要**：在HEK293细胞中表达重组ZFAND6蛋白，证实其通过激活Nrf2抗氧化通路减少神经元氧化损伤，提示在神经退行性疾病中的保护机制。
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注：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台以关键词“ZFAND6 recombinant protein”检索。建议结合近期研究(如2020年后)获取最新进展。
ZFAND6 (Zinc Finger AN1-Type Domain-Containing Protein 6) is a member of the AN1 zinc finger protein family, characterized by conserved zinc-binding motifs involved in protein-protein or protein-nucleic acid interactions. This protein contains two AN1-type zinc finger domains at its N-terminus and a C-terminal A20-like zinc finger domain, which are critical for its regulatory functions. ZFAND6 is implicated in cellular stress responses, apoptosis, and ubiquitination pathways, potentially acting as a modulator of proteasomal degradation or autophagy under stress conditions. Studies suggest its role in cancer biology, where it may influence tumor progression by regulating pathways linked to cell survival and proliferation. However, its precise molecular mechanisms remain under investigation.
Recombinant ZFAND6 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce purified protein for functional studies. The recombinant form typically retains key structural domains, enabling researchers to analyze its interactions with binding partners (e.g., ubiquitin ligases or proteasome components) and assess its impact on cellular processes. Its production often involves affinity tags (e.g., His-tag) for efficient purification and detection. Applications include *in vitro* assays to dissect signaling pathways, antibody development, and high-throughput screening for drug discovery. The protein’s role in stress adaptation and disease contexts, particularly cancer, makes it a subject of interest for therapeutic target validation. Despite progress, further research is needed to clarify its tissue-specific functions and regulatory networks.
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